TY  - JOUR
AU  - Thamm, M.
AU  - Balfanz, S.
AU  - Scheiner, R.
AU  - Baumann, A.
AU  - Blenau, W.
TI  - Characterization of the 5-HT(1A) receptor of the honeybee (Apis mellifera) and involvement of serotonin in phototactic behavior
JO  - Cellular and molecular life sciences
VL  - 67
SN  - 1420-682X
CY  - Basel
PB  - Birkhäuser
M1  - PreJuSER-10029
SP  - 2467 - 2479
PY  - 2010
N1  - We thank J. Schlenstedt for initiating the work on the honeybee 5-HT<INF>1A</INF> receptor and J. Erber for supporting the phototaxis experiments. This work was supported by the German Science Foundation (Research Training Group 837 Functional Insect Science, scholarship to M.T.).
AB  - Serotonin plays a key role in modulating various physiological and behavioral processes in both protostomes and deuterostomes. The vast majority of serotonin receptors belong to the superfamily of G-protein-coupled receptors. We report the cloning of a cDNA from the honeybee (Am5-ht1A) sharing high similarity with members of the 5-HT(1) receptor class. Activation of Am5-HT(1A) by serotonin inhibited the production of cAMP in a dose-dependent manner (EC(50) = 16.9 nM). Am5-HT(1A) was highly expressed in brain regions known to be involved in visual information processing. Using in vivo pharmacology, we could demonstrate that Am5-HT(1A) receptor ligands had a strong impact on the phototactic behavior of individual bees. The data presented here mark the first comprehensive study-from gene to behavior-of a 5-HT(1A) receptor in the honeybee, paving the way for the eventual elucidation of additional roles of this receptor subtype in the physiology and behavior of this social insect.
KW  - Amino Acid Sequence
KW  - Animals
KW  - Bees: metabolism
KW  - Bees: physiology
KW  - Behavior, Animal: drug effects
KW  - Brain: metabolism
KW  - Humans
KW  - Immunohistochemistry
KW  - Insect Proteins: chemistry
KW  - Insect Proteins: genetics
KW  - Insect Proteins: immunology
KW  - Insect Proteins: metabolism
KW  - Ligands
KW  - Light
KW  - Molecular Sequence Data
KW  - Movement: drug effects
KW  - Mushroom Bodies: metabolism
KW  - Optic Lobe, Nonmammalian: metabolism
KW  - Prazosin: pharmacology
KW  - Receptor, Serotonin, 5-HT1A: chemistry
KW  - Receptor, Serotonin, 5-HT1A: genetics
KW  - Receptor, Serotonin, 5-HT1A: immunology
KW  - Receptor, Serotonin, 5-HT1A: metabolism
KW  - Serotonin: analogs & derivatives
KW  - Serotonin: metabolism
KW  - Serotonin: pharmacology
KW  - Serotonin Antagonists: pharmacology
KW  - Serotonin Receptor Agonists: pharmacology
KW  - Insect Proteins (NLM Chemicals)
KW  - Ligands (NLM Chemicals)
KW  - Serotonin Antagonists (NLM Chemicals)
KW  - Serotonin Receptor Agonists (NLM Chemicals)
KW  - Receptor, Serotonin, 5-HT1A (NLM Chemicals)
KW  - Prazosin (NLM Chemicals)
KW  - Serotonin (NLM Chemicals)
KW  - 5-carboxamidotryptamine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20349263
UR  - <Go to ISI:>//WOS:000279025400010
DO  - DOI:10.1007/s00018-010-0350-6
UR  - https://juser.fz-juelich.de/record/10029
ER  -