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@ARTICLE{Thamm:10029,
author = {Thamm, M. and Balfanz, S. and Scheiner, R. and Baumann, A.
and Blenau, W.},
title = {{C}haracterization of the 5-{HT}(1{A}) receptor of the
honeybee ({A}pis mellifera) and involvement of serotonin in
phototactic behavior},
journal = {Cellular and molecular life sciences},
volume = {67},
issn = {1420-682X},
address = {Basel},
publisher = {Birkhäuser},
reportid = {PreJuSER-10029},
pages = {2467 - 2479},
year = {2010},
note = {We thank J. Schlenstedt for initiating the work on the
honeybee 5-HT<INF>1A</INF> receptor and J. Erber for
supporting the phototaxis experiments. This work was
supported by the German Science Foundation (Research
Training Group 837 Functional Insect Science, scholarship to
M.T.).},
abstract = {Serotonin plays a key role in modulating various
physiological and behavioral processes in both protostomes
and deuterostomes. The vast majority of serotonin receptors
belong to the superfamily of G-protein-coupled receptors. We
report the cloning of a cDNA from the honeybee (Am5-ht1A)
sharing high similarity with members of the 5-HT(1) receptor
class. Activation of Am5-HT(1A) by serotonin inhibited the
production of cAMP in a dose-dependent manner (EC(50) = 16.9
nM). Am5-HT(1A) was highly expressed in brain regions known
to be involved in visual information processing. Using in
vivo pharmacology, we could demonstrate that Am5-HT(1A)
receptor ligands had a strong impact on the phototactic
behavior of individual bees. The data presented here mark
the first comprehensive study-from gene to behavior-of a
5-HT(1A) receptor in the honeybee, paving the way for the
eventual elucidation of additional roles of this receptor
subtype in the physiology and behavior of this social
insect.},
keywords = {Amino Acid Sequence / Animals / Bees: metabolism / Bees:
physiology / Behavior, Animal: drug effects / Brain:
metabolism / Humans / Immunohistochemistry / Insect
Proteins: chemistry / Insect Proteins: genetics / Insect
Proteins: immunology / Insect Proteins: metabolism / Ligands
/ Light / Molecular Sequence Data / Movement: drug effects /
Mushroom Bodies: metabolism / Optic Lobe, Nonmammalian:
metabolism / Prazosin: pharmacology / Receptor, Serotonin,
5-HT1A: chemistry / Receptor, Serotonin, 5-HT1A: genetics /
Receptor, Serotonin, 5-HT1A: immunology / Receptor,
Serotonin, 5-HT1A: metabolism / Serotonin: analogs $\&$
derivatives / Serotonin: metabolism / Serotonin:
pharmacology / Serotonin Antagonists: pharmacology /
Serotonin Receptor Agonists: pharmacology / Insect Proteins
(NLM Chemicals) / Ligands (NLM Chemicals) / Serotonin
Antagonists (NLM Chemicals) / Serotonin Receptor Agonists
(NLM Chemicals) / Receptor, Serotonin, 5-HT1A (NLM
Chemicals) / Prazosin (NLM Chemicals) / Serotonin (NLM
Chemicals) / 5-carboxamidotryptamine (NLM Chemicals) / J
(WoSType)},
cin = {ISB-1},
ddc = {570},
cid = {I:(DE-Juel1)VDB922},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK505},
shelfmark = {Biochemistry $\&$ Molecular Biology / Cell Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20349263},
UT = {WOS:000279025400010},
doi = {10.1007/s00018-010-0350-6},
url = {https://juser.fz-juelich.de/record/10029},
}