%0 Journal Article
%A Götz, Jan
%A Wieters, Frederique
%A Fritz, Veronika J.
%A Käsgen, Olivia
%A Kalantari, Aref
%A Fink, Gereon Rudolf
%A Aswendt, Markus
%T Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice
%J Genes
%V 14
%N 2
%@ 2073-4425
%C Basel
%I MDPI
%M FZJ-2023-01614
%P 454 -
%D 2023
%X Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J mice using photothrombosis and performed qPCR on selected brain areas at 14, 28, and 56 days post-stroke (P14-56). Based on the grid walk and rotating beam test, the mice were classified into two groups. The expression of cAMP pathway genes Adora2a, Pde10a, and Drd2, was higher in poor- compared to well-recovered mice in contralesional primary motor cortex (cl-MOp) at P14&56 and cl-thalamus (cl-TH), but lower in cl-striatum (cl-Str) at P14 and cl-primary somatosensory cortex (cl-SSp) at P28. Plasticity and axonal sprouting genes, Lingo1 and BDNF, were decreased in cl-MOp at P14 and cl-Str at P28 and increased in cl-SSp at P28 and cl-Str at P14, respectively. In the cl-TH, Lingo1 was increased, and BDNF decreased at P14. Atrx, also involved in axonal sprouting, was only increased in poor-recovered mice in cl-MOp at P28. The results underline the gene expression dynamics and spatial variability and challenge existing theories of restricted neural plasticity.Keywords: behavior; cAMP pathway; grid walk; qPCR; recovery rate; rotating beam test.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 36833381
%U <Go to ISI:>//WOS:000939287700001
%R 10.3390/genes14020454
%U https://juser.fz-juelich.de/record/1005760