TY  - JOUR
AU  - Götz, Jan
AU  - Wieters, Frederique
AU  - Fritz, Veronika J.
AU  - Käsgen, Olivia
AU  - Kalantari, Aref
AU  - Fink, Gereon Rudolf
AU  - Aswendt, Markus
TI  - Temporal and Spatial Gene Expression Profile of Stroke Recovery Genes in Mice
JO  - Genes
VL  - 14
IS  - 2
SN  - 2073-4425
CY  - Basel
PB  - MDPI
M1  - FZJ-2023-01614
SP  - 454 -
PY  - 2023
AB  - Stroke patients show some degree of spontaneous functional recovery, but this is not sufficient to prevent long-term disability. One promising approach is to characterize the dynamics of stroke recovery genes in the lesion and distant areas. We induced sensorimotor cortex lesions in adult C57BL/6J mice using photothrombosis and performed qPCR on selected brain areas at 14, 28, and 56 days post-stroke (P14-56). Based on the grid walk and rotating beam test, the mice were classified into two groups. The expression of cAMP pathway genes Adora2a, Pde10a, and Drd2, was higher in poor- compared to well-recovered mice in contralesional primary motor cortex (cl-MOp) at P14&56 and cl-thalamus (cl-TH), but lower in cl-striatum (cl-Str) at P14 and cl-primary somatosensory cortex (cl-SSp) at P28. Plasticity and axonal sprouting genes, Lingo1 and BDNF, were decreased in cl-MOp at P14 and cl-Str at P28 and increased in cl-SSp at P28 and cl-Str at P14, respectively. In the cl-TH, Lingo1 was increased, and BDNF decreased at P14. Atrx, also involved in axonal sprouting, was only increased in poor-recovered mice in cl-MOp at P28. The results underline the gene expression dynamics and spatial variability and challenge existing theories of restricted neural plasticity.Keywords: behavior; cAMP pathway; grid walk; qPCR; recovery rate; rotating beam test.
LB  - PUB:(DE-HGF)16
C6  - 36833381
UR  - <Go to ISI:>//WOS:000939287700001
DO  - DOI:10.3390/genes14020454
UR  - https://juser.fz-juelich.de/record/1005760
ER  -