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001006587 0247_ $$2doi$$a10.1016/j.str.2023.03.008
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001006587 0247_ $$2datacite_doi$$a10.34734/FZJ-2023-01728
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001006587 1001_ $$aEvans, Sasha L.$$b0
001006587 245__ $$aSingle-particle cryo-EM analysis of the shell architecture and internal organization of an intact α-carboxysome
001006587 260__ $$aCambridge, Mass.$$bCell Press$$c2023
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001006587 520__ $$aCarboxysomes are proteinaceous bacterial microcompartments that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating several enzymes, including ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for the first step of the Calvin-Benson-Bassham cycle. Despite their significance in carbon fixation and great bioengineering potentials, the structural understanding of native carboxysomes is currently limited to low-resolution studies. Here, we report the characterization of a native α-carboxysome from a marine cyanobacterium by single-particle cryoelectron microscopy (cryo-EM). We have determined the structure of its RuBisCO enzyme, and obtained low-resolution maps of its icosahedral shell, and of its concentric interior organization. Using integrative modeling approaches, we have proposed a complete atomic model of an intact carboxysome, providing insight into its organization and assembly. This is critical for a better understanding of the carbon fixation mechanism and toward repurposing carboxysomes in synthetic biology for biotechnological applications.
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001006587 7001_ $$aAl-Hazeem, Monsour M. J.$$b1
001006587 7001_ $$0P:(DE-Juel1)179550$$aMann, Daniel$$b2$$ufzj
001006587 7001_ $$0P:(DE-HGF)0$$aSmetacek, Nicolas$$b3
001006587 7001_ $$0P:(DE-HGF)0$$aBeavil, Andrew J.$$b4
001006587 7001_ $$aSun, Yaqi$$b5
001006587 7001_ $$aChen, Taiyu$$b6
001006587 7001_ $$aDykes, Gregory F.$$b7
001006587 7001_ $$0P:(DE-Juel1)176583$$aLiu, Lu-Ning$$b8$$eCorresponding author
001006587 7001_ $$00000-0002-2841-9511$$aBergeron, Julien R. C.$$b9$$eCorresponding author
001006587 773__ $$0PERI:(DE-600)2031189-8$$a10.1016/j.str.2023.03.008$$gp. S0969212623000849$$pS0969212623000849$$tStructure$$v31$$x0969-2126$$y2023
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