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@ARTICLE{Schwanemann:1006798,
      author       = {Schwanemann, Tobias and Otto, Maike and Wynands, Benedikt
                      and Marienhagen, Jan and Wierckx, Nick},
      title        = {{A} {P}seudomonas taiwanensis {M}alonyl-{C}o{A} platform
                      strain for polyketide synthesis},
      journal      = {Metabolic engineering},
      volume       = {77},
      issn         = {1096-7176},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2023-01851},
      pages        = {219-230},
      year         = {2023},
      note         = {Biotechnologie 1},
      abstract     = {Malonyl-CoA is a central precursor for biosynthesis of a
                      wide range of complex secondary metabolites. The development
                      of platform strains with increased malonyl-CoA supply can
                      contribute to the efficient production of secondary
                      metabolites, especially if such strains exhibit high
                      tolerance towards these chemicals. In this study,
                      Pseudomonas taiwanensis VLB120 was engineered for increased
                      malonyl-CoA availability to produce bacterial and
                      plant-derived polyketides. A multi-target metabolic
                      engineering strategy focusing on decreasing the malonyl-CoA
                      drain and increasing malonyl-CoA precursor availability, led
                      to an increased production of various malonyl-CoA-derived
                      products, including pinosylvin, resveratrol and flaviolin.
                      The production of flaviolin, a molecule deriving from five
                      malonyl-CoA molecules, was doubled compared to the parental
                      strain by this malonyl-CoA increasing strategy.
                      Additionally, the engineered platform strain enabled
                      production of up to 84 mg L−1 resveratrol from
                      supplemented p-coumarate. One key finding of this study was
                      that acetyl-CoA carboxylase overexpression majorly
                      contributed to an increased malonyl-CoA availability for
                      polyketide production in dependence on the used
                      strain-background and whether downstream fatty acid
                      synthesis was impaired, reflecting its complexity in
                      metabolism. Hence, malonyl-CoA availability is primarily
                      determined by competition of the production pathway with
                      downstream fatty acid synthesis, while supply reactions are
                      of secondary importance for compounds that derive directly
                      from malonyl-CoA in Pseudomonas.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37031949},
      UT           = {WOS:001027176900001},
      doi          = {10.1016/j.ymben.2023.04.001},
      url          = {https://juser.fz-juelich.de/record/1006798},
}