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@ARTICLE{Junglas:1006880,
author = {Junglas, Benedikt and Gewehr, Lucas and Jilly, Ruven and
Franz, Johannes and Zu, Whenju Eva and Weidner, Tobias and
Bonn, Mischa and Sachse, Carsten and Schneider, Dirk},
title = {{S}yn{DLP} is a dynamin-like protein of {S}ynechocystis sp.
{PCC} 6803 with eukaryotic features},
journal = {Nature Communications},
volume = {14},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {FZJ-2023-01912},
pages = {2156},
year = {2023},
abstract = {Dynamin-like proteins are membrane remodeling GTPases with
well-understood functions in eukaryotic cells. However,
bacterial dynamin-like proteins are still poorly
investigated. SynDLP, the dynamin-like protein of the
cyanobacterium Synechocystis sp. PCC 6803, forms ordered
oligomers in solution. The 3.7 Å resolution cryo-EM
structure of SynDLP oligomers reveals the presence of
oligomeric stalk interfaces typical for eukaryotic
dynamin-like proteins. The bundle signaling element domain
shows distinct features, such as an intramolecular disulfide
bridge that affects the GTPase activity, or an expanded
intermolecular interface with the GTPase domain. In addition
to typical GD-GD contacts, such atypical GTPase domain
interfaces might be a GTPase activity regulating tool in
oligomerized SynDLP. Furthermore, we show that SynDLP
interacts with and intercalates into membranes containing
negatively charged thylakoid membrane lipids independent of
nucleotides. The structural characteristics of SynDLP
oligomers suggest it to be the closest known bacterial
ancestor of eukaryotic dynamin.},
cin = {ER-C-3},
ddc = {500},
cid = {I:(DE-Juel1)ER-C-3-20170113},
pnm = {5352 - Understanding the Functionality of Soft Matter and
Biomolecular Systems (POF4-535) / 5241 - Molecular
Information Processing in Cellular Systems (POF4-524)},
pid = {G:(DE-HGF)POF4-5352 / G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {37059718},
UT = {WOS:000969250300022},
doi = {10.1038/s41467-023-37746-9},
url = {https://juser.fz-juelich.de/record/1006880},
}