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@ARTICLE{Bannach:1007358,
      author       = {Bannach, Oliver and Pils, Marlene and Dybala, Alexandra and
                      Rehn, Fabian and Blömeke, Lara and Bujnicki, Tuyen and
                      Kraemer-Schulien, Victoria Maria and Hoyer, Wolfgang and
                      Riesner, Detlev and Willbold, Dieter},
      title        = {{D}evelopment and implementation of an internal quality
                      control sample to standardize oligomer-based diagnostics of
                      {A}lzheimer's disease},
      journal      = {Diagnostics},
      volume       = {13},
      number       = {10},
      issn         = {2075-4418},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2023-02027},
      pages        = {1702 -},
      year         = {2023},
      abstract     = {Protein misfolding and aggregation are pathological
                      hallmarks of various neurodegenerative diseases. In
                      Alzheimer’s disease (AD), soluble and toxic amyloid-β
                      (Aβ) oligomers are biomarker candidates for diagnostics and
                      drug development. However, accurate quantification of Aβ
                      oligomers in bodily fluids is challenging because extreme
                      sensitivity and specificity are required. We previously
                      introduced surface-based fluorescence intensity distribution
                      analysis (sFIDA) with single-particle sensitivity. In this
                      report, a preparation protocol for a synthetic Aβ oligomer
                      sample was developed. This sample was used for internal
                      quality control (IQC) to improve standardization, quality
                      assurance, and routine application of oligomer-based
                      diagnostic methods. We established an aggregation protocol
                      for Aβ1–42, characterized the oligomers by atomic force
                      microscopy (AFM), and assessed their application in sFIDA.
                      Globular-shaped oligomers with a median size of 2.67 nm were
                      detected by AFM, and sFIDA analysis of the Aβ1–42
                      oligomers yielded a femtomolar detection limit with high
                      assay selectivity and dilution linearity over 5 log units.
                      Lastly, we implemented a Shewhart chart for monitoring IQC
                      performance over time, which is another important step
                      toward quality assurance of oligomer-based diagnostic
                      methods.},
      cin          = {IBI-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37238187},
      UT           = {WOS:000998096700001},
      doi          = {10.3390/diagnostics13101702},
      url          = {https://juser.fz-juelich.de/record/1007358},
}