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@ARTICLE{Rbsam:1007690,
author = {Rübsam, Matthias and Püllen, Robin and Tellkamp, Frederik
and Bianco, Alessandra and Peskoller, Marc and Bloch,
Wilhelm and Green, Kathleen J. and Merkel, Rudolf and
Hoffmann, Bernd and Wickström, Sara A. and Niessen, Carien
M.},
title = {{P}olarity signaling balances epithelial contractility and
mechanical resistance},
journal = {Scientific reports},
volume = {13},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2023-02164},
pages = {7743},
year = {2023},
abstract = {Epithelia maintain a functional barrier during tissue
turnover while facing varying mechanical stress. This
maintenance requires both dynamic cell rearrangements driven
by actomyosin-linked intercellular adherens junctions and
ability to adapt to and resist extrinsic mechanical forces
enabled by keratin filament-linked desmosomes. How these two
systems crosstalk to coordinate cellular movement and
mechanical resilience is not known. Here we show that in
stratifying epithelia the polarity protein aPKCλ controls
the reorganization from stress fibers to cortical actomyosin
during differentiation and upward movement of cells. Without
aPKC, stress fibers are retained resulting in increased
contractile prestress. This aberrant stress is
counterbalanced by reorganization and bundling of keratins,
thereby increasing mechanical resilience. Inhibiting
contractility in aPKCλ−/− cells restores normal
cortical keratin networks but also normalizes resilience.
Consistently, increasing contractile stress is sufficient to
induce keratin bundling and enhance resilience, mimicking
aPKC loss. In conclusion, our data indicate that keratins
sense the contractile stress state of stratified epithelia
and balance increased contractility by mounting a protective
response to maintain tissue integrity.},
cin = {IBI-2},
ddc = {600},
cid = {I:(DE-Juel1)IBI-2-20200312},
pnm = {5243 - Information Processing in Distributed Systems
(POF4-524) / DFG project 273723265 - Mechanosensation und
Mechanoreaktion in epidermalen Systemen},
pid = {G:(DE-HGF)POF4-5243 / G:(GEPRIS)273723265},
typ = {PUB:(DE-HGF)16},
pubmed = {37173371},
UT = {WOS:000992578000016},
doi = {10.1038/s41598-023-33485-5},
url = {https://juser.fz-juelich.de/record/1007690},
}