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@ARTICLE{Babaki:1008453,
author = {Babaki, Mehrnaz and Fedosov, Dmitry A. and Gholivand,
Amirreza and Opdam, Joeri and Tuinier, Remco and Lettinga,
Minne Paul},
title = {{C}ompetition between deformation and free volume
quantified by 3{D} image analysis of red blood cell},
journal = {Biophysical journal},
volume = {122},
number = {9},
issn = {0006-3495},
address = {Bethesda, Md.},
publisher = {Soc.},
reportid = {FZJ-2023-02353},
pages = {1646 - 1658},
year = {2023},
abstract = {Cells in living organisms are subjected to mechanical
strains caused by external forces like overcrowding,
resulting in strong deformations that affect cell function.
We study the interplay between deformation and crowding of
red blood cells (RBCs) in dispersions of nonabsorbing
rod-like viruses. We identify a sequence of configurational
transitions of RBC doublets, including configurations that
can only be induced by long-ranged attraction: highly
fluctuating T-shaped and face-to-face configurations at low,
and doublets approaching a complete spherical configuration
at high, rod concentrations. Complementary simulations are
used to explore different energy contributions to
deformation as well as the stability of RBC doublet
configurations. Our advanced analysis of 3D reconstructed
confocal images of RBC doublets quantifies the depletion
interaction and the resulting deformation energy. Thus, we
introduce a noninvasive, high-throughput platform that is
generally applicable to investigate the mechanical response
of biological cells to external forces and characterize
their mechanical properties.},
cin = {IBI-4},
ddc = {570},
cid = {I:(DE-Juel1)IBI-4-20200312},
pnm = {5243 - Information Processing in Distributed Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5243},
typ = {PUB:(DE-HGF)16},
pubmed = {36964658},
UT = {WOS:000992419800001},
doi = {10.1016/j.bpj.2023.03.030},
url = {https://juser.fz-juelich.de/record/1008453},
}