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@ARTICLE{BacaCabrera:1009075,
author = {Baca Cabrera, Juan C. and Hirl, Regina T. and Zhu, Jianjun
and Schäufele, Rudi and Ogée, Jérôme and Schnyder, Hans},
title = {18 {O} enrichment of sucrose and photosynthetic and
nonphotosynthetic leaf water in a {C} 3 grass—atmospheric
drivers and physiological relations},
journal = {Plant, cell $\&$ environment},
volume = {46},
number = {9},
issn = {0140-7791},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2023-02623},
pages = {2628-2648},
year = {2023},
abstract = {The 18O enrichment (Δ18O) of leaf water affects the Δ18O
of photosynthetic products such as sucrose, generating an
isotopic archive of plant function and past climate.
However, uncertainty remains as to whether leaf water
compartmentation between photosynthetic and
nonphotosynthetic tissue affects the relationship between
Δ18O of bulk leaf water (Δ18OLW) and leaf sucrose
(Δ18OSucrose). We grew Lolium perenne (a C3 grass) in
mesocosm-scale, replicated experiments with daytime relative
humidity $(50\%$ or $75\%)$ and CO2 level (200, 400 or
800 μmol mol−1) as factors, and determined Δ18OLW,
Δ18OSucrose and morphophysiological leaf parameters,
including transpiration (Eleaf), stomatal conductance (gs)
and mesophyll conductance to CO2 (gm). The Δ18O of
photosynthetic medium water (Δ18OSSW) was estimated from
Δ18OSucrose and the equilibrium fractionation between water
and carbonyl groups (εbio). Δ18OSSW was well predicted by
theoretical estimates of leaf water at the evaporative site
(Δ18Oe) with adjustments that correlated with gas exchange
parameters (gs or total conductance to CO2). Isotopic mass
balance and published work indicated that nonphotosynthetic
tissue water was a large fraction (~0.53) of bulk leaf
water. Δ18OLW was a poor proxy for Δ18OSucrose, mainly due
to opposite Δ18O responses of nonphotosynthetic tissue
water (Δ18Onon-SSW) relative to Δ18OSSW, driven by
atmospheric conditions.},
cin = {IBG-3},
ddc = {580},
cid = {I:(DE-Juel1)IBG-3-20101118},
pnm = {2173 - Agro-biogeosystems: controls, feedbacks and impact
(POF4-217)},
pid = {G:(DE-HGF)POF4-2173},
typ = {PUB:(DE-HGF)16},
pubmed = {37376738},
UT = {WOS:001017555500001},
doi = {10.1111/pce.14655},
url = {https://juser.fz-juelich.de/record/1009075},
}