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@ARTICLE{Tan:1009340,
      author       = {Tan, Hua and Bungert-Plümke, Stefanie and Kortzak, Daniel
                      and Fahlke, Christoph and Stölting, Gabriel},
      title        = {{D}ual-color {C}olocalization in {S}ingle-molecule
                      {L}ocalization {M}icroscopy to {D}etermine the {O}ligomeric
                      {S}tate of {P}roteins in the {P}lasma {M}embrane},
      journal      = {Bio-protocol},
      volume       = {13},
      number       = {13},
      issn         = {2331-8325},
      address      = {Sunnyvale, CA},
      publisher    = {bio-protocol.org},
      reportid     = {FZJ-2023-02769},
      pages        = {4749},
      year         = {2023},
      note         = {Development of this protocol was supported by the Deutsche
                      Forschungsgemeinschaft (FA 301/15-1 to Ch.F.) as part of
                      Research Unit FOR 5046, project P4. We acknowledge the
                      original research paper (Tan et al., 2022), from which this
                      protocol is derived, as the primary reference when
                      additional information is needed.},
      abstract     = {Determining the oligomeric state of membrane proteins is
                      critical for understanding their function. However,
                      traditional ex situ methods like clear native gel
                      electrophoresis can disrupt protein subunit interactions
                      during sample preparation. In situ methods such as stepwise
                      photobleaching have limitations due to high expression
                      levels and limitations of optical resolution in microscopy.
                      Super-resolution microscopy techniques such as
                      single-molecule localization microscopy (SMLM) have the
                      potential to overcome these limitations, but the stochastic
                      nature of signals can lead to miscounting due to
                      over-expression, background noise, and temporal separation
                      of signals. Additionally, this technique has limited
                      application due to the limited selection of fluorescent
                      labels and the demanding control of laser power. To address
                      these issues, we developed a dual color colocalization (DCC)
                      strategy that offers higher tolerance to background noise
                      and simplifies data acquisition and processing for
                      high-throughput and reliable counting. The DCC strategy was
                      used to determine the oligomeric states of membrane proteins
                      of the SLC17 and SLC26 family with SMLM, providing a robust
                      and efficient method for studying protein interactions.},
      cin          = {IBI-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-1-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37456335},
      UT           = {WOS:001089325800011},
      doi          = {10.21769/BioProtoc.4749},
      url          = {https://juser.fz-juelich.de/record/1009340},
}