% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Steinhoff:1010189,
      author       = {Steinhoff, Heiko and Finger, Maurice and Osthege, Michael
                      and Golze, Corinna and Schito, Simone and Noack, Stephan and
                      Büchs, Jochen and Grünberger, Alexander},
      title        = {{E}xperimental k {S} estimation: {A} comparison of methods
                      for {C}orynebacterium glutamicum from lab to microfluidic
                      scale},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {120},
      number       = {5},
      issn         = {0368-1467},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2023-03000},
      pages        = {1288 - 1302},
      year         = {2023},
      abstract     = {Knowledge about the specific affinity of whole cells toward
                      a substrate, commonly referred to as kS, is a crucial
                      parameter for characterizing growth within bioreactors.
                      State-of-the-art methodologies measure either uptake or
                      consumption rates at different initial substrate
                      concentrations. Alternatively, cell dry weight or
                      respiratory data like online oxygen and carbon dioxide
                      transfer rates can be used to estimate kS. In this work, a
                      recently developed substrate-limited microfluidic
                      single-cell cultivation (sl-MSCC) method is applied for the
                      estimation of kS values under defined environmental
                      conditions. This method is benchmarked with two alternative
                      microtiter plate methods, namely high-frequency biomass
                      measurement (HFB) and substrate-limited respiratory activity
                      monitoring (sl-RA). As a model system, the substrate
                      affinity kS of Corynebacterium glutamicum ATCC 13032
                      regarding glucose was investigated assuming a Monod-type
                      growth response. A kS of <70.7 mg/L (with $95\%$
                      probability) with HFB, 8.55 ± 1.38 mg/L with sl-RA,
                      and 2.66 ± 0.99 mg/L with sl-MSCC was obtained.
                      Whereas HFB and sl-RA are suitable for a fast initial kS
                      estimation, sl-MSCC allows an affinity estimation by
                      determining tD at concentrations less or equal to the kS
                      value. Thus, sl-MSCC lays the foundation for strain-specific
                      kS estimations under defined environmental conditions with
                      additional insights into cell-to-cell heterogeneity.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217) / DFG project 427899901 -
                      Filamentöse Co-kulturen: vom Screening zur
                      Bioprozessentwicklung (Co-Pilot) (427899901) / DFG project
                      427904493 - Communities of niche-optimized strains (CoNoS)
                      – Ein neues Konzept zur Verbesserung der
                      biotechnologischen Produktion von kleinen Molekülen
                      (427904493)},
      pid          = {G:(DE-HGF)POF4-2171 / G:(GEPRIS)427899901 /
                      G:(GEPRIS)427904493},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {36740737},
      UT           = {WOS:000941292000001},
      doi          = {10.1002/bit.28345},
      url          = {https://juser.fz-juelich.de/record/1010189},
}