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| Hauptseite > Publikationsdatenbank > Experimental k S estimation: A comparison of methods for Corynebacterium glutamicum from lab to microfluidic scale > print |
| Hauptseite > Publikationsdatenbank > Experimental k S estimation: A comparison of methods for Corynebacterium glutamicum from lab to microfluidic scale > print |
| 001 | 1010189 | ||
| 005 | 20231027114412.0 | ||
| 024 | 7 | _ | |a 10.1002/bit.28345 |2 doi |
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| 037 | _ | _ | |a FZJ-2023-03000 |
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| 100 | 1 | _ | |a Steinhoff, Heiko |0 0000-0003-0536-2134 |b 0 |
| 245 | _ | _ | |a Experimental k S estimation: A comparison of methods for Corynebacterium glutamicum from lab to microfluidic scale |
| 260 | _ | _ | |a New York, NY [u.a.] |c 2023 |b Wiley |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1692255855_17360 |2 PUB:(DE-HGF) |
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| 520 | _ | _ | |a Knowledge about the specific affinity of whole cells toward a substrate, commonly referred to as kS, is a crucial parameter for characterizing growth within bioreactors. State-of-the-art methodologies measure either uptake or consumption rates at different initial substrate concentrations. Alternatively, cell dry weight or respiratory data like online oxygen and carbon dioxide transfer rates can be used to estimate kS. In this work, a recently developed substrate-limited microfluidic single-cell cultivation (sl-MSCC) method is applied for the estimation of kS values under defined environmental conditions. This method is benchmarked with two alternative microtiter plate methods, namely high-frequency biomass measurement (HFB) and substrate-limited respiratory activity monitoring (sl-RA). As a model system, the substrate affinity kS of Corynebacterium glutamicum ATCC 13032 regarding glucose was investigated assuming a Monod-type growth response. A kS of <70.7 mg/L (with 95% probability) with HFB, 8.55 ± 1.38 mg/L with sl-RA, and 2.66 ± 0.99 mg/L with sl-MSCC was obtained. Whereas HFB and sl-RA are suitable for a fast initial kS estimation, sl-MSCC allows an affinity estimation by determining tD at concentrations less or equal to the kS value. Thus, sl-MSCC lays the foundation for strain-specific kS estimations under defined environmental conditions with additional insights into cell-to-cell heterogeneity. |
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| 536 | _ | _ | |a DFG project 427899901 - Filamentöse Co-kulturen: vom Screening zur Bioprozessentwicklung (Co-Pilot) (427899901) |0 G:(GEPRIS)427899901 |c 427899901 |x 1 |
| 536 | _ | _ | |a DFG project 427904493 - Communities of niche-optimized strains (CoNoS) – Ein neues Konzept zur Verbesserung der biotechnologischen Produktion von kleinen Molekülen (427904493) |0 G:(GEPRIS)427904493 |c 427904493 |x 2 |
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| 700 | 1 | _ | |a Finger, Maurice |0 0000-0001-7384-8625 |b 1 |
| 700 | 1 | _ | |a Osthege, Michael |0 P:(DE-Juel1)174594 |b 2 |u fzj |
| 700 | 1 | _ | |a Golze, Corinna |0 P:(DE-HGF)0 |b 3 |
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| 700 | 1 | _ | |a Noack, Stephan |0 P:(DE-Juel1)129050 |b 5 |
| 700 | 1 | _ | |a Büchs, Jochen |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Grünberger, Alexander |0 P:(DE-Juel1)143612 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/bit.28345 |g Vol. 120, no. 5, p. 1288 - 1302 |0 PERI:(DE-600)1480809-2 |n 5 |p 1288 - 1302 |t Biotechnology & bioengineering |v 120 |y 2023 |x 0368-1467 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1010189/files/Biotech%20Bioengineering%20-%202023%20-%20Steinhoff.pdf |y OpenAccess |
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