%0 Journal Article
%A Byvaltcev, Egor
%A Behbood, Mahraz
%A Schleimer, Jan-Hendrik
%A Gensch, Thomas
%A Semyanov, Alexey
%A Schreiber, Susanne
%A Strauss, Ulf
%T KCC2 reverse mode helps to clear postsynaptically released potassium at glutamatergic synapses
%J Cell reports
%V 42
%N 8
%@ 2211-1247
%C [New York, NY]
%I Elsevier
%M FZJ-2023-03081
%P 112934 -
%D 2023
%Z This project has received funding from the EuropeanResearch Council (ERC) under the Union’s Horizon 2020 research an innovation program (grant agreement no. 864243) and from the Einstein FoundationBerlin (EP-2021-621).
%X Extracellular potassium [K+]o elevation during synaptic activity retrogradely modifies presynaptic release and astrocytic uptake of glutamate. Hence, local K+ clearance and replenishment mechanisms are crucial regulators of glutamatergic transmission and plasticity. Based on recordings of astrocytic inward rectifier potassium current IKir and K+-sensitive electrodes as sensors of [K+]o as well as on in silico modeling, we demonstrate that the neuronal K+-Cl- co-transporter KCC2 clears local perisynaptic [K+]o during synaptic excitation by operating in an activity-dependent reversed mode. In reverse mode, KCC2 replenishes K+ in dendritic spines and complements clearance of [K+]o, therewith attenuating presynaptic glutamate release and shortening LTP. We thus demonstrate a physiological role of KCC2 in neuron-glial interactions and regulation of synaptic signaling and plasticity through the uptake of postsynaptically released K+.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 37537840
%U <Go to ISI:>//WOS:001051595700001
%R 10.1016/j.celrep.2023.112934
%U https://juser.fz-juelich.de/record/1010486