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@ARTICLE{Fan:1010488,
author = {Fan, Xiaoming and Tao, Lele and Zhou, Xiaoyu and He, Xiao
and Zhang, Yu and Huang, Haixin and Yang, Jiale and Wang,
Simei and Ma, Zhihui and Gensch, Thomas and Huang, Ruimin},
title = {{M}ethod for the refractive index of various tissues based
on fluorescence microscopy},
journal = {Optics continuum},
volume = {2},
number = {7},
issn = {2770-0208},
address = {Washington, DC},
publisher = {Optica Publishing Group},
reportid = {FZJ-2023-03083},
pages = {1638 -},
year = {2023},
note = {National Natural Science Foundation of China (82172001);
Instrument Function Development of ChineseAcademy of
Sciences; Natural Science Foundation of Shanghai
(21ZR1474800).},
abstract = {Refractive index is an important optical constant that
characterizes the interactionbetween light and specimen. A
difference in refractive index between specimen and
immersionmedium introduces the imaging aberration and leads
to a problem that the direct thicknessmeasurement of a
specimen by optical microscopy is not accurate. However,
this aberrationcorrection still requires the exact
information of the refractive index of specimen and
immersionmedium. Herein, we propose an imaging strategy to
estimate the refractive index for an unknownspecimen. A
simplified diffraction model is generated to obtain the
relationship between axialscaling factor and refractive
index. Then regular fluorescence microscopy is performed to
measurethe actual axial scaling factors of specimens from
mouse muscle and tumor xenograft. Referringto our
theoretical plot of axial scaling factor versus refractive
index, the refractive index of tissuespecimen is determined.
For example, we obtain a mean refractive index (n) value of
1.36 fornormal muscle tissues, and 1.41 for tumor
xenografts. We demonstrate that this diffractionmodel-based
estimation method is an alternative to the current
techniques, improving the accuratemeasurement for refractive
index of tissue specimen. The simple instrument requirement
with aneasy specimen preparation for this estimation method
of refractive index may increase the imagequality on tissue
specimens with less aberration.},
cin = {IBI-1},
ddc = {530},
cid = {I:(DE-Juel1)IBI-1-20200312},
pnm = {5244 - Information Processing in Neuronal Networks
(POF4-524)},
pid = {G:(DE-HGF)POF4-5244},
typ = {PUB:(DE-HGF)16},
UT = {WOS:001044896900010},
doi = {10.1364/OPTCON.492897},
url = {https://juser.fz-juelich.de/record/1010488},
}