guest ::
| Home > Publications database > Method for the refractive index of various tissues based on fluorescence microscopy > print |
| Home > Publications database > Method for the refractive index of various tissues based on fluorescence microscopy > print |
| 001 | 1010488 | ||
| 005 | 20240226075454.0 | ||
| 024 | 7 | _ | |a 10.1364/OPTCON.492897 |2 doi |
| 024 | 7 | _ | |a 10.34734/FZJ-2023-03083 |2 datacite_doi |
| 024 | 7 | _ | |a WOS:001044896900010 |2 WOS |
| 037 | _ | _ | |a FZJ-2023-03083 |
| 082 | _ | _ | |a 530 |
| 100 | 1 | _ | |a Fan, Xiaoming |0 P:(DE-Juel1)144531 |b 0 |
| 245 | _ | _ | |a Method for the refractive index of various tissues based on fluorescence microscopy |
| 260 | _ | _ | |a Washington, DC |c 2023 |b Optica Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1706513446_21453 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a National Natural Science Foundation of China (82172001); Instrument Function Development of ChineseAcademy of Sciences; Natural Science Foundation of Shanghai (21ZR1474800). |
| 520 | _ | _ | |a Refractive index is an important optical constant that characterizes the interactionbetween light and specimen. A difference in refractive index between specimen and immersionmedium introduces the imaging aberration and leads to a problem that the direct thicknessmeasurement of a specimen by optical microscopy is not accurate. However, this aberrationcorrection still requires the exact information of the refractive index of specimen and immersionmedium. Herein, we propose an imaging strategy to estimate the refractive index for an unknownspecimen. A simplified diffraction model is generated to obtain the relationship between axialscaling factor and refractive index. Then regular fluorescence microscopy is performed to measurethe actual axial scaling factors of specimens from mouse muscle and tumor xenograft. Referringto our theoretical plot of axial scaling factor versus refractive index, the refractive index of tissuespecimen is determined. For example, we obtain a mean refractive index (n) value of 1.36 fornormal muscle tissues, and 1.41 for tumor xenografts. We demonstrate that this diffractionmodel-based estimation method is an alternative to the current techniques, improving the accuratemeasurement for refractive index of tissue specimen. The simple instrument requirement with aneasy specimen preparation for this estimation method of refractive index may increase the imagequality on tissue specimens with less aberration. |
| 536 | _ | _ | |a 5244 - Information Processing in Neuronal Networks (POF4-524) |0 G:(DE-HGF)POF4-5244 |c POF4-524 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Tao, Lele |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Zhou, Xiaoyu |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a He, Xiao |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Zhang, Yu |b 4 |
| 700 | 1 | _ | |a Huang, Haixin |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Yang, Jiale |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Wang, Simei |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Ma, Zhihui |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Gensch, Thomas |0 P:(DE-Juel1)131924 |b 9 |u fzj |
| 700 | 1 | _ | |a Huang, Ruimin |0 0000-0001-9369-0263 |b 10 |e Corresponding author |
| 773 | _ | _ | |a 10.1364/OPTCON.492897 |g Vol. 2, no. 7, p. 1638 - |0 PERI:(DE-600)3143831-3 |n 7 |p 1638 - |t Optics continuum |v 2 |y 2023 |x 2770-0208 |
| 856 | 4 | _ | |y OpenAccess |u https://juser.fz-juelich.de/record/1010488/files/optcon-2-7-1638-1.pdf |
| 856 | 4 | _ | |y OpenAccess |x icon |u https://juser.fz-juelich.de/record/1010488/files/optcon-2-7-1638-1.gif?subformat=icon |
| 856 | 4 | _ | |y OpenAccess |x icon-1440 |u https://juser.fz-juelich.de/record/1010488/files/optcon-2-7-1638-1.jpg?subformat=icon-1440 |
| 856 | 4 | _ | |y OpenAccess |x icon-180 |u https://juser.fz-juelich.de/record/1010488/files/optcon-2-7-1638-1.jpg?subformat=icon-180 |
| 856 | 4 | _ | |y OpenAccess |x icon-640 |u https://juser.fz-juelich.de/record/1010488/files/optcon-2-7-1638-1.jpg?subformat=icon-640 |
| 909 | C | O | |o oai:juser.fz-juelich.de:1010488 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 9 |6 P:(DE-Juel1)131924 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l Natural, Artificial and Cognitive Information Processing |1 G:(DE-HGF)POF4-520 |0 G:(DE-HGF)POF4-524 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-500 |4 G:(DE-HGF)POF |v Molecular and Cellular Information Processing |9 G:(DE-HGF)POF4-5244 |x 0 |
| 914 | 1 | _ | |y 2023 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2023-08-24 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |d 2023-03-08T13:54:12Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |d 2023-03-08T13:54:12Z |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b DOAJ : Anonymous peer review |d 2023-03-08T13:54:12Z |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2023-08-24 |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0112 |2 StatID |b Emerging Sources Citation Index |d 2023-08-24 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2023-08-24 |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)IBI-1-20200312 |k IBI-1 |l Molekular- und Zellphysiologie |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)IBI-1-20200312 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|