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@ARTICLE{UnverrichtYeboah:1010515,
      author       = {Unverricht-Yeboah, Marcus and Holtmann, Kathrin and
                      Kriehuber, Ralf},
      title        = {{C}omet {A}ssay analysis of {DNA} strand breaks after
                      exposure to the {DNA}-incorporated {A}uger {E}lectron
                      {E}mitter {I}odine-125},
      journal      = {International journal of radiation biology},
      volume       = {99},
      number       = {1},
      issn         = {0020-7616},
      address      = {Abingdon},
      publisher    = {Informa Healthcare},
      reportid     = {FZJ-2023-03098},
      pages        = {64 - 69},
      year         = {2023},
      abstract     = {Purpose: Ionizing radiation causes various types of DNA
                      damage e.g. single strand breaks (SSB) and double strand
                      breaks (DSB), whereby the SSB/DSB ratio is shifted toward
                      the DSB with increasing LET. For the DNA-incorporated Auger
                      electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is
                      calculated based on computer simulations. In the presented
                      work the SSB/DSB ratio of DNA-incorporated Iodine-125 was
                      experimentally determined and compared to external
                      homogenous γ-irradiation.Materials and methods:
                      Iodine-125-iododeoxyuridine (I-125-UdR) was incorporated
                      into the DNA of SCL-II cells and cells were subsequently
                      frozen for decay accumulation. Accordingly, external
                      γ-irradiation (Cs-137) experiments were performed in frozen
                      cells. After exposure the neutral or alkaline Comet Assay
                      was performed to quantify DSB or DSB and SSB, respectively.
                      Automated quantification of the comets was performed using
                      the Olive Tail Moment (Metafer CometScan; MetaSystems).
                      Calculation of absorbed dose for Auger electrons on cellular
                      level is extremely biased due to the exclusive DNA
                      localization of I-125-UdR. To avoid dose calculation the
                      γ-H2AX assay was used in order to allow the comparison of
                      the Comet Assay data between both investigated radiation
                      qualities.Results: For low-LET γ-radiation, a SSB/DSB ratio
                      of 10:1 was determined. In contrast, a lower SSB/DSB ratio
                      of 6:1 was induced by DNA-incorporated Iodine-125 which
                      compares very well to the calculated values of Pomplun and
                      co-authors.Conclusion: DNA-incorporated Iodine-125 induces a
                      high-LET type DNA damage pattern in respect to SSB/DSB
                      ratio.Keywords: Auger Electron Emitter; Comet Assay;
                      Iodine-125; high-LET type DNA damage},
      cin          = {S-US},
      ddc          = {570},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF4-899) / Verbundprojekt COLLAR:
                      Komplexe DNA-Läsionen und deren Bedeutung für die
                      zelluläre Antwort nach Bestrahlung, Teilprojekt A
                      (02NUK043A)},
      pid          = {G:(DE-HGF)POF4-899 / G:(BMBF)02NUK043A},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33211630},
      UT           = {WOS:000596176800001},
      doi          = {10.1080/09553002.2020.1851059},
      url          = {https://juser.fz-juelich.de/record/1010515},
}