%0 Journal Article
%A Kriehuber, Ralf
%A Riedling, Manuela
%A Simk�, Myrtill
%A Weiss, Dieter G.
%T Cytotoxicity, genotoxicity and intracellular distribution of the Auger electron emitter 65 Zn in two human cell lines
%J Radiation and environmental biophysics
%V 43
%N 1
%@ 0006-3517
%C New York, NY
%I Springer
%M FZJ-2023-03101
%P 15 - 22
%D 2004
%X Cell survival, induction of apoptosis, and micronucleus formation have been examined in non-transformed human amnion fluid fibroblast-like (AFFL) cells and in a human squameous cell carcinoma (SCL-II) cell line after exposure to the Auger electron emitter (65)Zn and after external low-LET radiation. Cellular uptake and subcellular distribution of (65)Zn(2+) were studied in vitro and the absorbed radiation dose was calculated applying analytical dosimetry models. Auger electrons generated during decay of (65)Zn induced a prominent decrease in cell survival and increased the levels of apoptotic as well as micronucleated cells when compared to external low-LET irradiation. Relative biological effectiveness has been determined for cell survival (RBE approximately 4), micronucleus formation (RBE approximately 2) and apoptosis induction (RBE approximately 5-8) in SCL-II cells and for micronucleus formation (RBE approximately 4-5) and apoptosis induction (RBE approximately 6-10) in AFFL cells, respectively. This demonstrates a general enhanced biological effectiveness of (65)Zn in both investigated cell lines when compared to external low-LET radiation. The distribution pattern of intracellular Zn(2+) was found to be non-uniform, showing enhanced amounts of Zn(2+) in the perinuclear region and low amounts inside the cell nucleus, suggesting a major energy deposition close to the nuclear envelope.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 15106009
%U <Go to ISI:>//WOS:000221192400003
%R 10.1007/s00411-004-0234-y
%U https://juser.fz-juelich.de/record/1010518