TY  - JOUR
AU  - Kriehuber, Ralf
AU  - Riedling, Manuela
AU  - Simk�, Myrtill
AU  - Weiss, Dieter G.
TI  - Cytotoxicity, genotoxicity and intracellular distribution of the Auger electron emitter 65 Zn in two human cell lines
JO  - Radiation and environmental biophysics
VL  - 43
IS  - 1
SN  - 0006-3517
CY  - New York, NY
PB  - Springer
M1  - FZJ-2023-03101
SP  - 15 - 22
PY  - 2004
AB  - Cell survival, induction of apoptosis, and micronucleus formation have been examined in non-transformed human amnion fluid fibroblast-like (AFFL) cells and in a human squameous cell carcinoma (SCL-II) cell line after exposure to the Auger electron emitter (65)Zn and after external low-LET radiation. Cellular uptake and subcellular distribution of (65)Zn(2+) were studied in vitro and the absorbed radiation dose was calculated applying analytical dosimetry models. Auger electrons generated during decay of (65)Zn induced a prominent decrease in cell survival and increased the levels of apoptotic as well as micronucleated cells when compared to external low-LET irradiation. Relative biological effectiveness has been determined for cell survival (RBE approximately 4), micronucleus formation (RBE approximately 2) and apoptosis induction (RBE approximately 5-8) in SCL-II cells and for micronucleus formation (RBE approximately 4-5) and apoptosis induction (RBE approximately 6-10) in AFFL cells, respectively. This demonstrates a general enhanced biological effectiveness of (65)Zn in both investigated cell lines when compared to external low-LET radiation. The distribution pattern of intracellular Zn(2+) was found to be non-uniform, showing enhanced amounts of Zn(2+) in the perinuclear region and low amounts inside the cell nucleus, suggesting a major energy deposition close to the nuclear envelope.
LB  - PUB:(DE-HGF)16
C6  - 15106009
UR  - <Go to ISI:>//WOS:000221192400003
DO  - DOI:10.1007/s00411-004-0234-y
UR  - https://juser.fz-juelich.de/record/1010518
ER  -