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@ARTICLE{Kriehuber:1010518,
author = {Kriehuber, Ralf and Riedling, Manuela and Simk�, Myrtill
and Weiss, Dieter G.},
title = {{C}ytotoxicity, genotoxicity and intracellular distribution
of the {A}uger electron emitter 65 {Z}n in two human cell
lines},
journal = {Radiation and environmental biophysics},
volume = {43},
number = {1},
issn = {0006-3517},
address = {New York, NY},
publisher = {Springer},
reportid = {FZJ-2023-03101},
pages = {15 - 22},
year = {2004},
abstract = {Cell survival, induction of apoptosis, and micronucleus
formation have been examined in non-transformed human amnion
fluid fibroblast-like (AFFL) cells and in a human squameous
cell carcinoma (SCL-II) cell line after exposure to the
Auger electron emitter (65)Zn and after external low-LET
radiation. Cellular uptake and subcellular distribution of
(65)Zn(2+) were studied in vitro and the absorbed radiation
dose was calculated applying analytical dosimetry models.
Auger electrons generated during decay of (65)Zn induced a
prominent decrease in cell survival and increased the levels
of apoptotic as well as micronucleated cells when compared
to external low-LET irradiation. Relative biological
effectiveness has been determined for cell survival (RBE
approximately 4), micronucleus formation (RBE approximately
2) and apoptosis induction (RBE approximately 5-8) in SCL-II
cells and for micronucleus formation (RBE approximately 4-5)
and apoptosis induction (RBE approximately 6-10) in AFFL
cells, respectively. This demonstrates a general enhanced
biological effectiveness of (65)Zn in both investigated cell
lines when compared to external low-LET radiation. The
distribution pattern of intracellular Zn(2+) was found to be
non-uniform, showing enhanced amounts of Zn(2+) in the
perinuclear region and low amounts inside the cell nucleus,
suggesting a major energy deposition close to the nuclear
envelope.},
cin = {S-US},
ddc = {530},
cid = {I:(DE-Juel1)S-US-20090406},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {15106009},
UT = {WOS:000221192400003},
doi = {10.1007/s00411-004-0234-y},
url = {https://juser.fz-juelich.de/record/1010518},
}