%0 Journal Article
%A Kriehuber, Ralf
%A Simko, M
%A Schiffmann, D.
%A Trott, K.-R.
%T Delayed cytotoxic and genotoxic effects in a human cell line following X-irradiation
%J International journal of radiation biology
%V 75
%N 8
%@ 0020-7616
%C Abingdon
%I Informa Healthcare
%M FZJ-2023-03103
%P 1021 - 1027
%D 1999
%X Background: In order to clarify the relationship between delayed reproductive death and radiation-induced genomic instability, the colony-forming efficiency of surviving, irradiated human squamous carcinoma cells and centromere positive as well as centromere negative micronuclei in surviving progeny were examined.Materials and methods: Colony-forming ability and micronucleus (MN) frequency in binucleated cells 24 h after the addition of cytochalasin B during 2 weeks of post-irradiation growth were determined in a squamous cell carcinoma cell line (SCL-II) of human origin. In addition, centromeres in micronuclei were detected using FISH.Results: In the human epithelial cell line used for these experiments, delayed reproductive death was pronounced and persisted for at least 2 weeks after irradiation. Although there is evidence for an increased rate of centromere positive micronuclei, but not of centromere negative micronuclei, arising during the first week of post-irradiation proliferation, this decreases later while the rate of delayed reproductive death remains elevated.Conclusion: In the studied cell line, the observed delayed reproductive death is not closely related to the investigated criteria of radiation-induced genomic instability. This casts doubt on the common assumption that delayed reproductive death is a direct manifestation of radiation-induced genomic instability.
%F PUB:(DE-HGF)16
%9 Journal Article
%R 10.1080/095530099139782
%U https://juser.fz-juelich.de/record/1010520