001010520 001__ 1010520
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001010520 0247_ $$2doi$$a10.1080/095530099139782
001010520 0247_ $$2ISSN$$a0020-7616
001010520 0247_ $$2ISSN$$a0955-3002
001010520 0247_ $$2ISSN$$a1362-3095
001010520 037__ $$aFZJ-2023-03103
001010520 041__ $$aEnglish
001010520 082__ $$a570
001010520 1001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b0$$eCorresponding author$$ufzj
001010520 245__ $$aDelayed cytotoxic and genotoxic effects in a human cell line following X-irradiation
001010520 260__ $$aAbingdon$$bInforma Healthcare$$c1999
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001010520 520__ $$aBackground: In order to clarify the relationship between delayed reproductive death and radiation-induced genomic instability, the colony-forming efficiency of surviving, irradiated human squamous carcinoma cells and centromere positive as well as centromere negative micronuclei in surviving progeny were examined.Materials and methods: Colony-forming ability and micronucleus (MN) frequency in binucleated cells 24 h after the addition of cytochalasin B during 2 weeks of post-irradiation growth were determined in a squamous cell carcinoma cell line (SCL-II) of human origin. In addition, centromeres in micronuclei were detected using FISH.Results: In the human epithelial cell line used for these experiments, delayed reproductive death was pronounced and persisted for at least 2 weeks after irradiation. Although there is evidence for an increased rate of centromere positive micronuclei, but not of centromere negative micronuclei, arising during the first week of post-irradiation proliferation, this decreases later while the rate of delayed reproductive death remains elevated.Conclusion: In the studied cell line, the observed delayed reproductive death is not closely related to the investigated criteria of radiation-induced genomic instability. This casts doubt on the common assumption that delayed reproductive death is a direct manifestation of radiation-induced genomic instability.
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001010520 7001_ $$0P:(DE-HGF)0$$aSimko, M$$b1
001010520 7001_ $$0P:(DE-HGF)0$$aSchiffmann, D.$$b2
001010520 7001_ $$0P:(DE-HGF)0$$aTrott, K.-R.$$b3
001010520 773__ $$0PERI:(DE-600)3065-X$$a10.1080/095530099139782$$gVol. 75, no. 8, p. 1021 - 1027$$n8$$p1021 - 1027$$tInternational journal of radiation biology$$v75$$x0020-7616$$y1999
001010520 8564_ $$uhttps://juser.fz-juelich.de/record/1010520/files/Delayed%20cytotoxic%20and%20genotoxic%20effects%20in%20a%20human%20cell%20line%20following%20X%20irradiation.pdf
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