TY  - JOUR
AU  - Kriehuber, Ralf
AU  - Simko, M
AU  - Schiffmann, D.
AU  - Trott, K.-R.
TI  - Delayed cytotoxic and genotoxic effects in a human cell line following X-irradiation
JO  - International journal of radiation biology
VL  - 75
IS  - 8
SN  - 0020-7616
CY  - Abingdon
PB  - Informa Healthcare
M1  - FZJ-2023-03103
SP  - 1021 - 1027
PY  - 1999
AB  - Background: In order to clarify the relationship between delayed reproductive death and radiation-induced genomic instability, the colony-forming efficiency of surviving, irradiated human squamous carcinoma cells and centromere positive as well as centromere negative micronuclei in surviving progeny were examined.Materials and methods: Colony-forming ability and micronucleus (MN) frequency in binucleated cells 24 h after the addition of cytochalasin B during 2 weeks of post-irradiation growth were determined in a squamous cell carcinoma cell line (SCL-II) of human origin. In addition, centromeres in micronuclei were detected using FISH.Results: In the human epithelial cell line used for these experiments, delayed reproductive death was pronounced and persisted for at least 2 weeks after irradiation. Although there is evidence for an increased rate of centromere positive micronuclei, but not of centromere negative micronuclei, arising during the first week of post-irradiation proliferation, this decreases later while the rate of delayed reproductive death remains elevated.Conclusion: In the studied cell line, the observed delayed reproductive death is not closely related to the investigated criteria of radiation-induced genomic instability. This casts doubt on the common assumption that delayed reproductive death is a direct manifestation of radiation-induced genomic instability.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1080/095530099139782
UR  - https://juser.fz-juelich.de/record/1010520
ER  -