TY  - JOUR
AU  - Radchuk, Volodymyr
AU  - Belew, Zeinu M
AU  - Gündel, Andre
AU  - Mayer, Simon
AU  - Hilo, Alexander
AU  - Hensel, Goetz
AU  - Sharma, Rajiv
AU  - Neumann, Kerstin
AU  - Ortleb, Stefan
AU  - Wagner, Steffen
AU  - Muszynska, Aleksandra
AU  - Crocoll, Christoph
AU  - Xu, Deyang
AU  - Hoffie, Iris
AU  - Kumlehn, Jochen
AU  - Fuchs, Joerg
AU  - Peleke, Fritz F
AU  - Szymanski, Jedrzej J
AU  - Rolletschek, Hardy
AU  - Nour-Eldin, Hussam H
AU  - Borisjuk, Ljudmilla
TI  - SWEET11b transports both sugar and cytokinin in developing barley grains
JO  - The plant cell
VL  - 35
IS  - 6
SN  - 1040-4651
CY  - Rockville, Md.
PB  - Soc.
M1  - FZJ-2023-03111
SP  - 2186 - 2207
PY  - 2023
AB  - Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not only just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome.
LB  - PUB:(DE-HGF)16
C6  - 36857316
UR  - <Go to ISI:>//WOS:000946526400001
DO  - DOI:10.1093/plcell/koad055
UR  - https://juser.fz-juelich.de/record/1010536
ER  -