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@ARTICLE{Helm:1014222,
      author       = {Helm, Tabea and Niehoff, Paul-Joachim and Gätgens, Jochem
                      and Stausberg, Thilo and Pichler, Bernadette and Häßler,
                      Thomas and Wiechert, Wolfgang and Büchs, Jochen and
                      Wierckx, Nick and Noack, Stephan},
      title        = {{I}ntroducing molasses as an alternative feedstock into
                      itaconate production using {U}stilago sp},
      journal      = {New biotechnology},
      volume       = {77},
      issn         = {1871-6784},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2023-03206},
      pages        = {30 - 39},
      year         = {2023},
      abstract     = {In this work, we established an efficient process for the
                      production of itaconate from the regionally sourced
                      industrial side-stream molasses using Ustilago cynodontis
                      and Ustilago maydis. While being relatively cheap and more
                      environmentally friendly than refined sugars, there are some
                      major challenges to overcome when working with molasses.
                      Some of those challenges are a high nitrogen load, unknown
                      impurities in the feedstock, and high amounts of
                      ill-favoured carbon sources, such as sucrose or lactate. We
                      could show that the activity of the sucrose-hydrolysing
                      enzyme invertase plays a crucial role in the efficiency of
                      the process and that the fructose utilisation differs
                      between the two strains used in this work. Thus, with a
                      higher invertase activity, the ability to convert fructose
                      into the desired product itaconate, and an overall higher
                      tolerance towards undesired substances in molasses, U.
                      maydis is better equipped for the process on the alternative
                      feedstock molasses than U. cynodontis. The established
                      process with U. maydis reached competitive yields of up to
                      0.38 g g−1 and a titre of more than 37 g L−1. This shows
                      that an efficient and cost-effective itaconate production
                      process is generally feasible using U. maydis, which has the
                      potential to greatly increase the sustainability of
                      industrial itaconate production.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37336283},
      UT           = {WOS:001038624600001},
      doi          = {10.1016/j.nbt.2023.06.003},
      url          = {https://juser.fz-juelich.de/record/1014222},
}