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| Hauptseite > Publikationsdatenbank > Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation > print |
| Hauptseite > Publikationsdatenbank > Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation > print |
| 001 | 1014224 | ||
| 005 | 20231023093621.0 | ||
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| 100 | 1 | _ | |a Stief, Tobias |0 P:(DE-Juel1)187436 |b 0 |
| 245 | _ | _ | |a Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2023 |b Elsevier |
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| 520 | _ | _ | |a The neuronal SNARE protein SNAP25a (isoform 2) forms part of the SNARE complex eliciting synaptic vesicle fusion during neuronal exocytosis. While the post-fusion cis-SNARE complex has been studied extensively, little is known about the pre-fusion conformation of SNAP25a. Here we analyze monomeric SNAP25a by NMR spectroscopy, further supported by small-angle X-ray scattering (SAXS) experiments. SAXS data indicate that monomeric SNAP25 is more compact than a Gaussian chain but still a random coil. NMR shows that for monomeric SNAP25a, before SNAP25a interacts with its SNARE partners to drive membrane fusion, only the N-terminal part (region A5 to V36) of the first SNARE motif, SN1 (L11 - L81), is helical, comprising two α-helices (ranging from A5 to Q20 and S25 toV36). From E37 onwards, SNAP25a is mostly disordered and displays high internal flexibility, including the C-terminal part of SN1, almost the entire second SNARE motif (SN2, N144-A199), and the connecting loop region. Apart from the N-terminal helices, only the C-termini of both SN1 (E73 - K79) and SN2 (region T190 - A199), as well as two short regions in the connecting loop (D99 - K102 and E123 - M127) show a weak α-helical propensity (α-helical population < 25%). We speculate that the N-terminal helices (A5 to Q20 and S25 to V36) which constitute the N-terminus of SN1 act as a nucleation site for initiating SNARE zippering. |
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| 773 | _ | _ | |a 10.1016/j.jmb.2023.168069 |g Vol. 435, no. 10, p. 168069 - |0 PERI:(DE-600)1355192-9 |n 10 |p 168069 - |t Journal of molecular biology |v 435 |y 2023 |x 0022-2836 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1014224/files/Stief_et_al_JMB_2023-1.pdf |
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