001014968 001__ 1014968
001014968 005__ 20231027114415.0
001014968 0247_ $$2doi$$a10.1038/s42004-023-00998-z
001014968 0247_ $$2datacite_doi$$a10.34734/FZJ-2023-03512
001014968 0247_ $$2pmid$$a37697032
001014968 0247_ $$2WOS$$aWOS:001066506600001
001014968 037__ $$aFZJ-2023-03512
001014968 082__ $$a540
001014968 1001_ $$00000-0003-2248-3544$$aPerez-Garcia, Pablo$$b0
001014968 245__ $$aAn archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate
001014968 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2023
001014968 3367_ $$2DRIVER$$aarticle
001014968 3367_ $$2DataCite$$aOutput Types/Journal article
001014968 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1695377610_3362
001014968 3367_ $$2BibTeX$$aARTICLE
001014968 3367_ $$2ORCID$$aJOURNAL_ARTICLE
001014968 3367_ $$00$$2EndNote$$aJournal Article
001014968 520__ $$aPolyethylene terephthalate (PET) is a commodity polymer known to globally contaminate marine and terrestrial environments. Today, around 80 bacterial and fungal PET-active enzymes (PETases) are known, originating from four bacterial and two fungal phyla. In contrast, no archaeal enzyme had been identified to degrade PET. Here we report on the structural and biochemical characterization of PET46 (RLI42440.1), an archaeal promiscuous feruloyl esterase exhibiting degradation activity on semi-crystalline PET powder comparable to IsPETase and LCC (wildtypes), and higher activity on bis-, and mono-(2-hydroxyethyl) terephthalate (BHET and MHET). The enzyme, found by a sequence-based metagenome search, is derived from a non-cultivated, deep-sea Candidatus Bathyarchaeota archaeon. Biochemical characterization demonstrated that PET46 is a promiscuous, heat-adapted hydrolase. Its crystal structure was solved at a resolution of 1.71 Å. It shares the core alpha/beta-hydrolase fold with bacterial PETases, but contains a unique lid common in feruloyl esterases, which is involved in substrate binding. Thus, our study widens the currently known diversity of PET-hydrolyzing enzymes, by demonstrating PET depolymerization by a plant cell wall-degrading esterase.
001014968 536__ $$0G:(DE-HGF)POF4-2171$$a2171 - Biological and environmental resources for sustainable use (POF4-217)$$cPOF4-217$$fPOF IV$$x0
001014968 536__ $$0G:(GEPRIS)417919780$$aDFG project 417919780 - Zentrum für strukturelle Studien (417919780)$$c417919780$$x1
001014968 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
001014968 7001_ $$00000-0002-7499-5325$$aChow, Jennifer$$b1
001014968 7001_ $$0P:(DE-HGF)0$$aCostanzi, Elisa$$b2
001014968 7001_ $$0P:(DE-HGF)0$$aGurschke, Marno$$b3
001014968 7001_ $$00000-0003-2377-2268$$aDittrich, Jonas$$b4
001014968 7001_ $$00000-0001-7438-8843$$aDierkes, Robert F.$$b5
001014968 7001_ $$0P:(DE-Juel1)171724$$aMolitor, Rebecka$$b6$$ufzj
001014968 7001_ $$0P:(DE-HGF)0$$aApplegate, Violetta$$b7
001014968 7001_ $$00000-0003-0741-1729$$aFeuerriegel, Golo$$b8
001014968 7001_ $$0P:(DE-HGF)0$$aTete, Prince$$b9
001014968 7001_ $$0P:(DE-HGF)0$$aDanso, Dominik$$b10
001014968 7001_ $$0P:(DE-Juel1)128936$$aThies, Stephan$$b11$$ufzj
001014968 7001_ $$0P:(DE-HGF)0$$aSchumacher, Julia$$b12
001014968 7001_ $$0P:(DE-HGF)0$$aPfleger, Christopher$$b13
001014968 7001_ $$0P:(DE-Juel1)131457$$aJaeger, Karl-Erich$$b14$$ufzj
001014968 7001_ $$0P:(DE-Juel1)172663$$aGohlke, Holger$$b15$$ufzj
001014968 7001_ $$00000-0003-0780-9251$$aSmits, Sander H. J.$$b16
001014968 7001_ $$00000-0002-6788-0829$$aSchmitz, Ruth A.$$b17$$eCorresponding author
001014968 7001_ $$00000-0001-7617-7396$$aStreit, Wolfgang R.$$b18$$eCorresponding author
001014968 773__ $$0PERI:(DE-600)2929562-2$$a10.1038/s42004-023-00998-z$$gVol. 6, no. 1, p. 193$$n1$$p193$$tCommunications chemistry$$v6$$x2399-3669$$y2023
001014968 8564_ $$uhttps://juser.fz-juelich.de/record/1014968/files/s42004-023-00998-z.pdf$$yOpenAccess
001014968 909CO $$ooai:juser.fz-juelich.de:1014968$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
001014968 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)171724$$aForschungszentrum Jülich$$b6$$kFZJ
001014968 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128936$$aForschungszentrum Jülich$$b11$$kFZJ
001014968 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131457$$aForschungszentrum Jülich$$b14$$kFZJ
001014968 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)172663$$aForschungszentrum Jülich$$b15$$kFZJ
001014968 9131_ $$0G:(DE-HGF)POF4-217$$1G:(DE-HGF)POF4-210$$2G:(DE-HGF)POF4-200$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-2171$$aDE-HGF$$bForschungsbereich Erde und Umwelt$$lErde im Wandel – Unsere Zukunft nachhaltig gestalten$$vFür eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten$$x0
001014968 9141_ $$y2023
001014968 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2022-11-30
001014968 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
001014968 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2022-11-30
001014968 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2022-11-30
001014968 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
001014968 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2022-11-30
001014968 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-05-02T09:13:12Z
001014968 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-05-02T09:13:12Z
001014968 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2023-05-02T09:13:12Z
001014968 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCOMMUN CHEM : 2022$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2023-10-27
001014968 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bCOMMUN CHEM : 2022$$d2023-10-27
001014968 920__ $$lyes
001014968 9201_ $$0I:(DE-Juel1)IBG-4-20200403$$kIBG-4$$lBioinformatik$$x0
001014968 9201_ $$0I:(DE-Juel1)IMET-20090612$$kIMET$$lInstitut für Molekulare Enzymtechnologie (HHUD)$$x1
001014968 980__ $$ajournal
001014968 980__ $$aVDB
001014968 980__ $$aUNRESTRICTED
001014968 980__ $$aI:(DE-Juel1)IBG-4-20200403
001014968 980__ $$aI:(DE-Juel1)IMET-20090612
001014968 9801_ $$aFullTexts