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| Hauptseite > Publikationsdatenbank > DNA-binding and protein structure of nuclear factors likely acting in genetic information processing in the Paulinella chromatophore > print |
| Hauptseite > Publikationsdatenbank > DNA-binding and protein structure of nuclear factors likely acting in genetic information processing in the Paulinella chromatophore > print |
| 001 | 1014978 | ||
| 005 | 20231023093629.0 | ||
| 024 | 7 | _ | |a 10.1073/pnas.2221595120 |2 doi |
| 024 | 7 | _ | |a 0027-8424 |2 ISSN |
| 024 | 7 | _ | |a 1091-6490 |2 ISSN |
| 024 | 7 | _ | |a 10.34734/FZJ-2023-03522 |2 datacite_doi |
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| 037 | _ | _ | |a FZJ-2023-03522 |
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| 100 | 1 | _ | |a Macorano, Luis |0 0009-0006-1979-3495 |b 0 |
| 245 | _ | _ | |a DNA-binding and protein structure of nuclear factors likely acting in genetic information processing in the Paulinella chromatophore |
| 260 | _ | _ | |a Washington, DC |c 2023 |b National Acad. of Sciences |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a The chromatophores in Paulinella are evolutionary-early-stage photosynthetic organelles. Biological processes in chromatophores depend on a combination of chromatophore and nucleus-encoded proteins. Interestingly, besides proteins carrying chromatophore-targeting signals, a large arsenal of short chromatophore-targeted proteins (sCTPs; <90 amino acids) without recognizable targeting signals were found in chromatophores. This situation resembles endosymbionts in plants and insects that are manipulated by host-derived antimicrobial peptides. Previously, we identified an expanded family of sCTPs of unknown function, named here "DNA-binding (DB)-sCTPs". DB-sCTPs contain a ~45 amino acid motif that is conserved in some bacterial proteins with predicted functions in DNA processing. Here, we explored antimicrobial activity, DNA-binding capacity, and structures of three purified recombinant DB-sCTPs. All three proteins exhibited antimicrobial activity against bacteria involving membrane permeabilization, and bound to bacterial lipids in vitro. A combination of in vitro assays demonstrated binding of recombinant DB-sCTPs to chromatophore-derived genomic DNA sequences with an affinity in the low nM range. Additionally, we report the 1.2 Å crystal structure of one DB-sCTP. In silico docking studies suggest that helix α2 inserts into the DNA major grove and the exposed residues, that are highly variable between different DB-sCTPs, confer interaction with the DNA bases. Identification of photosystem II subunit CP43 as a potential interaction partner of one DB-sCTP, suggests DB-sCTPs to be involved in more complex regulatory mechanisms. We hypothesize that membrane binding of DB-sCTPs is related to their import into chromatophores. Once inside, they interact with the chromatophore genome potentially providing nuclear control over genetic information processing. |
| 536 | _ | _ | |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524) |0 G:(DE-HGF)POF4-5241 |c POF4-524 |f POF IV |x 0 |
| 536 | _ | _ | |a SFB 1208 B09 - Erforschung der Kontaktzone zwischen Wirtszelle und ihren neu erworbenen photosynthetischen Organellen in der Amöbe Paulinella chromatophora (Rhizaria, Cercozoa) (B09) (289580405) |0 G:(GEPRIS)289580405 |c 289580405 |x 1 |
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| 700 | 1 | _ | |a Binny, Taniya M. |0 0000-0002-4043-4511 |b 1 |
| 700 | 1 | _ | |a Spiegl, Tobias |0 0000-0002-6701-7224 |b 2 |
| 700 | 1 | _ | |a Klimenko, Victoria |0 0009-0001-1777-1587 |b 3 |
| 700 | 1 | _ | |a Singer, Anna |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Oberleitner, Linda |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Applegate, Violetta |0 0000-0003-4120-7983 |b 6 |
| 700 | 1 | _ | |a Seyffert, Sarah |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Stefanski, Anja |0 0000-0001-8532-954X |b 8 |
| 700 | 1 | _ | |a Gremer, Lothar |0 P:(DE-Juel1)145165 |b 9 |
| 700 | 1 | _ | |a Gertzen, Christoph G. W. |b 10 |
| 700 | 1 | _ | |a Höppner, Astrid |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Smits, Sander H. J. |0 0000-0003-0780-9251 |b 12 |
| 700 | 1 | _ | |a Nowack, Eva C. M. |0 0000-0002-6920-6838 |b 13 |e Corresponding author |
| 773 | _ | _ | |a 10.1073/pnas.2221595120 |g Vol. 120, no. 27, p. e2221595120 |0 PERI:(DE-600)1461794-8 |n 27 |p e2221595120 |t Proceedings of the National Academy of Sciences of the United States of America |v 120 |y 2023 |x 0027-8424 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/1014978/files/pnas.2221595120.pdf |y OpenAccess |
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