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@INPROCEEDINGS{Lettinga:1017170,
author = {Lettinga, M.P. and Babaki, Mehrnaz and Gholivand, Amirreza
and Fedosov, Dmitry and Korculanin, Olivera and Tuinier,
Remco and Opdam, Joeri and Wagner, Christian},
title = {{C}ompetition between deformation and free volume
quantified by 3{D} image analysis of red blood cells },
reportid = {FZJ-2023-03983},
year = {2023},
abstract = {Cells in living organisms are subjected to mechanical
strains caused by external forces like overcrowding,
resulting in strong deformations that affect cell function.
We study the interplay between deformation and crowding of
red blood cells (RBCs) in dispersions of non-absorbing
rod-like viruses. We identify a sequence of configurational
transitions of RBC doublets, including configurations that
can only be induced by long-ranged attraction: highly
fluctuating T-shaped and face-to-face configurations at low,
and doublets approaching a complete spherical configuration
at high rod concentrations.Complementary simulations are
used to explore different energy contributions to
deformation as well as the stability of RBC doublet
configurations. Our advanced analysis of 3D reconstructed
confocal images of RBCs doublets quantifies the depletion
interaction and the resulting deformation energy. Thus, we
introduce a non-invasive method, high-throughput platform
that is generally applicable to investigate the mechanical
response of biological cells to external forces
andcharacterize their mechanical properties.},
month = {Jul},
date = {2023-07-10},
organization = {Dycap 2023, Compiegne (France), 10 Jul
2023 - 13 Jul 2023},
subtyp = {After Call},
cin = {IBI-4},
cid = {I:(DE-Juel1)IBI-4-20200312},
pnm = {5243 - Information Processing in Distributed Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5243},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/1017170},
}