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@ARTICLE{Kang:1017753,
author = {Kang, Kyongok},
title = {{T}he effect of fatty acids, ionic strength, and electric
fields on the microscopic dynamics of {BSA} aggregates},
journal = {Frontiers in physics},
volume = {11},
issn = {2296-424X},
address = {Lausanne},
publisher = {Frontiers Media},
reportid = {FZJ-2023-04289},
pages = {1282099},
year = {2023},
abstract = {This paper presents the microscopic dynamics of the
concentrated suspensions ofbovine serum albumin (BSA)
proteins and their aggregates by dynamic lightscattering
(DLS) experiments. The effects of fatty acids binding to
BSA, as wellas the ionic strength and weak electric field,
are discussed for affecting the stabilityof BSA suspensions
against calcium-induced aggregation. By variation of the
ionicstrength, in the absence of an external electric field,
DLS experiments show thatmonomer–BSA interactions (in the
essentially fatty acid-free case) are overallrepulsive but
that, nevertheless, aggregation occurs to some extent. Also,
thediffusive properties of different types of BSA are
explored under an applied low-ACelectric field by means of
in situ electric small-angle depolarized DLS
experiments,which reveal a significant decrease of the
translational BSA–monomer diffusioncoefficient with
increasing frequency, while the aggregates indicate
orientationalmotion via rotation on applying an electric
field. These observations areinterpreted in terms of
(localized) orientation interactions obtained asoscillations
in the intermediate scattering correlation function, as well
as theanomalous slower relaxations as resulting in effective
(collective) dynamicsbetween monomeric BSA and their protein
aggregates.},
cin = {IBI-4},
ddc = {530},
cid = {I:(DE-Juel1)IBI-4-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
UT = {WOS:001092372200001},
doi = {10.3389/fphy.2023.1282099},
url = {https://juser.fz-juelich.de/record/1017753},
}