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@ARTICLE{Wang:1018695,
      author       = {Wang, Jiali and Platz-Baudin, Eric and Noetzel-Reiss, Erik
                      and Offenhäusser, Andreas and Maybeck, Vanessa},
      title        = {{E}xpressing {O}ptogenetic {A}ctuators {F}used to
                      {N}-terminal {M}ucin {M}otifs {D}elivers {T}argets to
                      {S}pecific {S}ubcellular {C}ompartments in {P}olarized
                      {C}ells},
      journal      = {Advanced biology},
      volume       = {8},
      number       = {3},
      issn         = {2366-7478},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2023-04989},
      pages        = {2300428},
      year         = {2024},
      abstract     = {Optogenetics is a powerful approach in neuroscience
                      research. However, other tissues of the body may benefit
                      from controlled ion currents and neuroscience may benefit
                      from more precise optogenetic expression. The present work
                      constructs three subcellularly-targeted optogenetic
                      actuators based on the channelrhodopsin ChR2-XXL, utilizing
                      5, 10, or 15 tandem repeats (TR) from mucin as N-terminal
                      targeting motifs and evaluates expression in several
                      polarized and non-polarized cell types. The modified
                      channelrhodopsin maintains its electrophysiological
                      properties, which can be used to produce continuous membrane
                      depolarization, despite the expected size of the repeats.
                      This work then shows that these actuators are subcellularly
                      localized in polarized cells. In polarized epithelial cells,
                      all three actuators localize to just the lateral membrane.
                      The TR-tagged constructs also express subcellularly in
                      cortical neurons, where TR5-ChR2XXL and TR10-ChR2XXL mainly
                      target the somatodendrites. Moreover, the transfection
                      efficiencies are shown to be dependent on cell type and
                      tandem repeat length. Overall, this work verifies that the
                      targeting motifs from epithelial cells can be used to
                      localize optogenetic actuators in both epithelia and
                      neurons, opening epithelia processes to optogenetic
                      manipulation and providing new possibilities to target
                      optogenetic tools.},
      cin          = {IBI-3 / IBI-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-3-20200312 / I:(DE-Juel1)IBI-2-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38015104},
      UT           = {WOS:001119680100001},
      doi          = {10.1002/adbi.202300428},
      url          = {https://juser.fz-juelich.de/record/1018695},
}