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@MASTERSTHESIS{Robertz:1018978,
      author       = {Robertz, Stefan and Reinecke-Levi, Diana},
      othercontributors = {Janzik, Ingar},
      title        = {{C}haracterization of {C}hlorellales cell walls –
                      {S}patio-temporal refined biochemical and morphological
                      characterization of {C}hlorella sorokinianaand vulgaris, and
                      {P}arachlorella kessleri},
      school       = {Fachhochschule Aachen},
      type         = {Masterarbeit},
      publisher    = {Fachhochschule Aachen},
      reportid     = {FZJ-2023-05047},
      pages        = {109 p.},
      year         = {2021},
      note         = {Masterarbeit, Fachhochschule Aachen, 2021},
      abstract     = {Microalgae of the family Chlorellaceae are of special
                      interest to the food, feed, and nutraceutical industry, due
                      to their high content of valuable nutrients. However, the
                      bioaccessibility is limited by the rigid and polymorph cell
                      wall of these species. This work aimed to characterize the
                      cell walls of three Chlorellaceae strains in-vivo and
                      in-vitro under industry-related culturing conditions.
                      Histochemical staining and detailed analyses of cell wall
                      monosaccharide composition identified changes in the
                      composition during cell maturation in Chlorella sorokiniana
                      SAG 211-8k, Chlorella vulgaris SAG 211-11b and Parachlorella
                      kessleri SAG 211-11g. The cell walls of C. sorokiniana and
                      P. kessleri developed a higher hemicellulose content
                      (defined as susceptible to 2 mol/L trifluoroacetic acid
                      hydrolysis) with stable amounts of the recalcitrance
                      components (susceptible to 6 mol/L HCl). In contrast, the
                      cell wall of C. vulgaris incorporated increasingly
                      recalcitrance components and developed higher
                      robustness.Additionally, a novel endogenous cell-wall-lytic
                      enzyme (autologous Autolysin) was extracted from C.
                      sorokiniana and P. kessleri. It showed endo-β-1,3-Glucanase
                      activity, and partial autologous cell wall degradation in
                      both strains. Thus, presenting a first step towards more
                      effective methods for increased bioaccessibility, protoplast
                      formation and genetic manipulation of Chlorellaceae
                      strains.},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)19},
      doi          = {10.34734/FZJ-2023-05047},
      url          = {https://juser.fz-juelich.de/record/1018978},
}