001019308 001__ 1019308
001019308 005__ 20240222203104.0
001019308 0247_ $$2doi$$a10.1038/s41467-023-43460-3
001019308 0247_ $$2datacite_doi$$a10.34734/FZJ-2023-05282
001019308 0247_ $$2pmid$$a38057304
001019308 0247_ $$2WOS$$aWOS:001135959900008
001019308 037__ $$aFZJ-2023-05282
001019308 082__ $$a500
001019308 1001_ $$0P:(DE-Juel1)179550$$aMann, Daniel$$b0
001019308 245__ $$aAtg18 oligomer organization in assembled tubes and on lipid membrane scaffolds
001019308 260__ $$a[London]$$bNature Publishing Group UK$$c2023
001019308 3367_ $$2DRIVER$$aarticle
001019308 3367_ $$2DataCite$$aOutput Types/Journal article
001019308 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1708595059_12136
001019308 3367_ $$2BibTeX$$aARTICLE
001019308 3367_ $$2ORCID$$aJOURNAL_ARTICLE
001019308 3367_ $$00$$2EndNote$$aJournal Article
001019308 520__ $$aAutophagy-related protein 18 (Atg18) participates in the elongation of early autophagosomal structures in concert with Atg2 and Atg9 complexes. How Atg18 contributes to the structural coordination of Atg2 and Atg9 at the isolation membrane remains to be understood. Here, we determined the cryo-EM structures of Atg18 organized in helical tubes, Atg18 oligomers in solution as well as on lipid membrane scaffolds. The helical assembly is composed of Atg18 tetramers forming a lozenge cylindrical lattice with remarkable structural similarity to the COPII outer coat. When reconstituted with lipid membranes, using subtomogram averaging we determined tilted Atg18 dimer structures bridging two juxtaposed lipid membranes spaced apart by 80 Å. Moreover, lipid reconstitution experiments further delineate the contributions of Atg18's FRRG motif and the amphipathic helical extension in membrane interaction. The observed structural plasticity of Atg18's oligomeric organization and membrane binding properties provide a molecular framework for the positioning of downstream components of the autophagy machinery.
001019308 536__ $$0G:(DE-HGF)POF4-5352$$a5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535)$$cPOF4-535$$fPOF IV$$x0
001019308 536__ $$0G:(DE-HGF)POF4-5241$$a5241 - Molecular Information Processing in Cellular Systems (POF4-524)$$cPOF4-524$$fPOF IV$$x1
001019308 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
001019308 7001_ $$00000-0002-2094-1911$$aFromm, Simon A.$$b1
001019308 7001_ $$00000-0002-5865-2138$$aMartinez-Sanchez, Antonio$$b2
001019308 7001_ $$aGopaldass, Navin$$b3
001019308 7001_ $$aChoy, Ramona$$b4
001019308 7001_ $$00000-0001-6131-313X$$aMayer, Andreas$$b5
001019308 7001_ $$0P:(DE-Juel1)173949$$aSachse, Carsten$$b6$$eCorresponding author
001019308 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-023-43460-3$$gVol. 14, no. 1, p. 8086$$n1$$p8086$$tNature Communications$$v14$$x2041-1723$$y2023
001019308 8564_ $$uhttps://juser.fz-juelich.de/record/1019308/files/Atg18%20oligomer%2011.12.2023.pdf$$yOpenAccess
001019308 8564_ $$uhttps://juser.fz-juelich.de/record/1019308/files/Atg18%20oligomer%2011.12.2023.gif?subformat=icon$$xicon$$yOpenAccess
001019308 8564_ $$uhttps://juser.fz-juelich.de/record/1019308/files/Atg18%20oligomer%2011.12.2023.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
001019308 8564_ $$uhttps://juser.fz-juelich.de/record/1019308/files/Atg18%20oligomer%2011.12.2023.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
001019308 8564_ $$uhttps://juser.fz-juelich.de/record/1019308/files/Atg18%20oligomer%2011.12.2023.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
001019308 8767_ $$8SN-2023-00880-b$$92024-02-08$$d2024-02-14$$eAPC$$jZahlung erfolgt
001019308 8767_ $$8SN-2023-00880-b$$92024-02-08$$d2024-02-14$$eAPC$$jZahlung angewiesen
001019308 909CO $$ooai:juser.fz-juelich.de:1019308$$pdnbdelivery$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire
001019308 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)179550$$aForschungszentrum Jülich$$b0$$kFZJ
001019308 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)173949$$aForschungszentrum Jülich$$b6$$kFZJ
001019308 9131_ $$0G:(DE-HGF)POF4-535$$1G:(DE-HGF)POF4-530$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5352$$aDE-HGF$$bKey Technologies$$lMaterials Systems Engineering$$vMaterials Information Discovery$$x0
001019308 9131_ $$0G:(DE-HGF)POF4-524$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5241$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vMolecular and Cellular Information Processing$$x1
001019308 9141_ $$y2023
001019308 915pc $$0PC:(DE-HGF)0000$$2APC$$aAPC keys set
001019308 915pc $$0PC:(DE-HGF)0001$$2APC$$aLocal Funding
001019308 915pc $$0PC:(DE-HGF)0002$$2APC$$aDFG OA Publikationskosten
001019308 915pc $$0PC:(DE-HGF)0003$$2APC$$aDOAJ Journal
001019308 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-05-02T09:09:09Z
001019308 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT COMMUN : 2022$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-05-02T09:09:09Z
001019308 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
001019308 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-08-29
001019308 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
001019308 915__ $$0StatID:(DE-HGF)9915$$2StatID$$aIF >= 15$$bNAT COMMUN : 2022$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2023-05-02T09:09:09Z
001019308 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-08-29
001019308 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2023-08-29
001019308 920__ $$lyes
001019308 9201_ $$0I:(DE-Juel1)ER-C-3-20170113$$kER-C-3$$lStrukturbiologie$$x0
001019308 980__ $$ajournal
001019308 980__ $$aVDB
001019308 980__ $$aI:(DE-Juel1)ER-C-3-20170113
001019308 980__ $$aAPC
001019308 980__ $$aUNRESTRICTED
001019308 9801_ $$aAPC
001019308 9801_ $$aFullTexts