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@ARTICLE{Abuawad:1019313,
      author       = {Abuawad, Abdalhalim and Ashhab, Yaqoub and Offenhäusser,
                      Andreas and Krause, Hans-Joachim},
      title        = {{DNA} {S}ensor for the {D}etection of {B}rucella spp.
                      {B}ased on {M}agnetic {N}anoparticle {M}arkers},
      journal      = {International journal of molecular sciences},
      volume       = {24},
      number       = {24},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {FZJ-2023-05287},
      pages        = {17272 -},
      year         = {2023},
      abstract     = {Due to the limitations of conventional Brucella detection
                      methods, including safety concerns, long incubation times,
                      and limited specificity, the development of a rapid,
                      selective, and accu-rate technique for the early detection
                      of Brucella in livestock animals is crucial to prevent the
                      spread of the associated disease. In the present study, we
                      introduce a magnetic nanoparticle marker-based biosensor
                      using frequency mixing magnetic detection for point-of-care
                      testing and quantification of Brucella DNA.
                      Superparamagnetic nanoparticles were used as magnetically
                      measured markers to selectively detect the target DNA
                      hybridized with its complementary cap-ture probes
                      immobilized on a porous polyethylene filter. Experimental
                      conditions like density and length of the probes,
                      hybridization time and temperature, and magnetic binding
                      specificity, sensitivity, and detection limit were
                      investigated and optimized. Our sensor demonstrated a
                      relatively fast detection time of approximately 10 min, with
                      a detection limit of 55 copies (0.09 fM) when tested using
                      DNA amplified from Brucella genetic material. In addition,
                      the detection specificity was examined using gDNA from
                      Brucella and other zoonotic bacteria that may coexist in the
                      same niche, confirming the method’s selectivity for
                      Brucella DNA. Our proposed biosensor has the potential to be
                      used for the early detection of Brucella bacteria in the
                      field and can con-tribute to disease control measures.},
      cin          = {IBI-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {38139102},
      UT           = {WOS:001132844200001},
      doi          = {10.3390/ijms242417272},
      url          = {https://juser.fz-juelich.de/record/1019313},
}