001019314 001__ 1019314
001019314 005__ 20231219202011.0
001019314 037__ $$aFZJ-2023-05288
001019314 041__ $$aEnglish
001019314 1001_ $$0P:(DE-Juel1)128697$$aKrause, Hans-Joachim$$b0$$eCorresponding author$$ufzj
001019314 1112_ $$a4th European BioSensor Symposium 2023$$cAachen$$d2023-08-27 - 2023-08-30$$gEBS 2023$$wGermany
001019314 245__ $$aMagnetic Biosensing and Frequency Mixing Magnetic Detection
001019314 260__ $$c2023
001019314 3367_ $$033$$2EndNote$$aConference Paper
001019314 3367_ $$2DataCite$$aOther
001019314 3367_ $$2BibTeX$$aINPROCEEDINGS
001019314 3367_ $$2DRIVER$$aconferenceObject
001019314 3367_ $$2ORCID$$aLECTURE_SPEECH
001019314 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1702969089_18144$$xInvited
001019314 520__ $$aFor selective detection and quantification of biomolecular targets, we use magnetic label-based immunoassays employing the highly specific interaction between antigens and antibodies bound to magnetic nanoparticle (MNP) markers, which are measured by frequency mixing magnetic detection (FMMD). Superparamagnetic MNP exhibit a nonlinear magnetization. Upon magnetic excitation at two distinct frequencies f1 and f2 incident on the sample, the nonlinearity gives rise to the generation of intermodulation frequencies. Coaxial coils provide magnetic excitation at frequency f1 while the MNP are driven near magnetic saturation with a driving frequency incident on the sample. Response signals generated at linear combination frequencies f1 ± n×f2 are picked up by a detection coil, differentially connected to a reference coil, and are demodulated in the magnetic reader device. The appearance of the mixing components is highly specific to the nonlinearity of the magnetization curve of the particles. The method yields a very large dynamic range of detection, extending to more than 4 orders of magnitude in the number of particles. Analysis of the phase of the response gives information on the magnetic relaxation of the MNP, and thus on their hy-drodynamic size and binding state. Variation of excitation amplitudes or a static magnetic offset field enables determining the size distribution of the magnetic cores of the MNP. With the FMMD technique, magnetic sandwich immunoassays for various biomolecular targets have been successfully realized, exhibiting improved detection limits as compared to conventional techniques like ELISA.
001019314 536__ $$0G:(DE-HGF)POF4-5241$$a5241 - Molecular Information Processing in Cellular Systems (POF4-524)$$cPOF4-524$$fPOF IV$$x0
001019314 536__ $$0G:(GEPRIS)445454801$$aDFG project 445454801 - Kombinierter Feldeffekt-/Magnet-Immunsensor-Chip für den markierungsfreien Nachweise von Biomarkern (FEMIC) (445454801)$$c445454801$$x1
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001019314 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128697$$aForschungszentrum Jülich$$b0$$kFZJ
001019314 9131_ $$0G:(DE-HGF)POF4-524$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5241$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vMolecular and Cellular Information Processing$$x0
001019314 9141_ $$y2023
001019314 920__ $$lyes
001019314 9201_ $$0I:(DE-Juel1)IBI-3-20200312$$kIBI-3$$lBioelektronik$$x0
001019314 980__ $$aconf
001019314 980__ $$aVDB
001019314 980__ $$aI:(DE-Juel1)IBI-3-20200312
001019314 980__ $$aUNRESTRICTED