001019315 001__ 1019315
001019315 005__ 20231213202052.0
001019315 037__ $$aFZJ-2023-05289
001019315 041__ $$aEnglish
001019315 1001_ $$0P:(DE-Juel1)128697$$aKrause, Hans-Joachim$$b0$$eCorresponding author$$ufzj
001019315 1112_ $$aJCNS-2 Seminar$$cJülich$$wGermany
001019315 245__ $$aFrequency Mixing Magnetic Detection of Superparamagnetic Nanoparticles as Markers for Immunoassays$$f2023-11-09 - 
001019315 260__ $$c2023
001019315 3367_ $$033$$2EndNote$$aConference Paper
001019315 3367_ $$2DataCite$$aOther
001019315 3367_ $$2BibTeX$$aINPROCEEDINGS
001019315 3367_ $$2ORCID$$aLECTURE_SPEECH
001019315 3367_ $$0PUB:(DE-HGF)31$$2PUB:(DE-HGF)$$aTalk (non-conference)$$btalk$$mtalk$$s1702449886_7741$$xInvited
001019315 3367_ $$2DINI$$aOther
001019315 520__ $$aSuperparamagnetic Nanoparticles exhibit a nonlinear magnetization. Upon magnetic excitation at two distinct frequencies f1 and f2, the nonlinearity gives rise to the generation of response signals at intermodulation frequencies m×f1 ± n×f2 which are picked up by a detection coil and demodulated in a magnetic reader device. The appearance of the mixing components is highly specific to the nonlinearity of the magnetization curve of the particles. Preferably, the response signal at frequency f1 + 2×f2 is detected as it gives the strongest response. The method yields a very large dynamic range of detection, extending to more than 4 orders of magnitude in the number of particles. Analysis of the phase of the response gives information on the magnetic relaxation of the particles, and thus on their hydrodynamic size and binding state. Variation of excitation amplitudes or a static magnetic offset field enables determining the size distribution of the particles’ magnetic cores. Magnetic immunoassays for various biomolecular targets such as Cholera toxin B in water, antibiotics in milk, and SARS-CoV-2-specific antibodies in blood have been successfully realized. The magnetic reader enables portable immunodetection at point-of-care, with improved detection limits as compared to conventional lab-based enzyme linked immuno sorbent assays (ELISA).
001019315 536__ $$0G:(DE-HGF)POF4-5241$$a5241 - Molecular Information Processing in Cellular Systems (POF4-524)$$cPOF4-524$$fPOF IV$$x0
001019315 536__ $$0G:(GEPRIS)445454801$$aDFG project 445454801 - Kombinierter Feldeffekt-/Magnet-Immunsensor-Chip für den markierungsfreien Nachweise von Biomarkern (FEMIC) (445454801)$$c445454801$$x1
001019315 909CO $$ooai:juser.fz-juelich.de:1019315$$pVDB
001019315 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128697$$aForschungszentrum Jülich$$b0$$kFZJ
001019315 9131_ $$0G:(DE-HGF)POF4-524$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5241$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vMolecular and Cellular Information Processing$$x0
001019315 9141_ $$y2023
001019315 920__ $$lyes
001019315 9201_ $$0I:(DE-Juel1)IBI-3-20200312$$kIBI-3$$lBioelektronik$$x0
001019315 980__ $$atalk
001019315 980__ $$aVDB
001019315 980__ $$aI:(DE-Juel1)IBI-3-20200312
001019315 980__ $$aUNRESTRICTED