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@INPROCEEDINGS{Chlubek:1019677,
author = {Chlubek, Antonia and Pflugfelder, Daniel and Metzner, Ralf
and Huber, Gregor and Schurr, Ulrich and Hinz, Carsten and
Koller, Robert and Scheins, Jürgen and Streun, Matthias},
title = {{A} routine {C}arbon-11-{PET} workflow for in vivo
measurements of plants: from a custom plant facility to data
analysis},
reportid = {FZJ-2023-05534},
year = {2023},
abstract = {Positron Emission Tomography (PET) is a potent tool in
plant sciences to measure allocation and transport processes
in vivo. Especially the radioisotope Carbon-11 is of major
interest as it can be administered as 11CO2 to leaves and
used as a tracer to monitor the flow of photoassimilates
within complex 3D structures such as roots.However, in the
past, PET studies on plants have primarily been conducted on
clinical or preclinical PET scanners. These scanners have
the drawback of being horizontally orientated and typically
not optimized for a plant-friendly environment, including
suboptimal temperature, humidity and light conditions.
Studies conducted on plant dedicated scanners have often
lacked a full characterization, quantitative image
reconstruction (including attenuation correction of
individual samples) and data analysis pipeline.In this
poster we present the establishment of a complete routine
Carbon-11 PET pipeline for plants. This pipeline begins with
a custom PET facility for plants, which comprises the
production and administration of 11CO2, gas exchange
measurement, and a climate chamber with a fully
characterized and calibrated plant-dedicated PET scanner,
the phenoPET. It also includes workflow protocols to measure
up to four plants per day and ensures that data from
individual plants in measuring series can be reliably and
quantitatively compared. Additionally, the application of an
in-house developed compartmental model for long-distance
transport allows us to quantify and analyze flow velocities
of the tracer and exchange rates with other tissues along
the transport pathways, including error calculation.},
month = {Nov},
date = {2023-11-20},
organization = {2nd Workshop Carbon Allocation in
Plants, Versailles (France), 20 Nov
2023 - 21 Nov 2023},
subtyp = {Other},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2173 - Agro-biogeosystems: controls, feedbacks and impact
(POF4-217)},
pid = {G:(DE-HGF)POF4-2173},
typ = {PUB:(DE-HGF)24},
doi = {10.34734/FZJ-2023-05534},
url = {https://juser.fz-juelich.de/record/1019677},
}