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@INPROCEEDINGS{vanDusschoten:1020589,
      author       = {van Dusschoten, Dagmar and Pflugfelder, Daniel and Le Gall,
                      Samuel and Koller, Robert},
      title        = {{M}agnetic {R}esonance {I}maging of early wheat seedlings
                      and possible relevance for root water uptake},
      reportid     = {FZJ-2024-00284},
      year         = {2023},
      abstract     = {Seed germination and seedling establishment are the first
                      important steps in a plant's growing cycle. We optimized a
                      measurement sequence to quantify the early stages of root
                      development in young seedlings in natural soil. We used
                      magnetic resonance imaging (MRI), providing us with 3D
                      information about root structures non-invasively. By growing
                      up to 18 seedlings per pot (Ø=12.5cm, 10cm height) and by
                      focusing on early growth (up to 4 days after start of
                      germination), a much higher plant throughput compared to
                      traditional 3D root measurement protocols in soil was
                      achieved. Due to high temporal resolution of the acquired
                      data (4 images per day), dynamic traits such as shoot and
                      root emergence time were obtained accurately. We used this
                      'deep phenotyping' approach, with several temporal and
                      spatial layers of data, to investigate phenotypic
                      differences within the 8 parent lines of the NIAB MAGIC
                      population. Clear phenotypic differences in structural (e.g.
                      root angle, root lengths, and number) and temporal (e.g.
                      time of root emergence, shoot emergence) were quantified.
                      The initial root angle may be important for rooting depth at
                      later stages which can potentially influence root water
                      uptake (RWU) depth profiles. We show preliminary data using
                      our home-build Soil Water Profiler (SWaP) on localized RWU
                      for wheat plants in relatively wet soil and compare these
                      with results we found for other species. This new MRI
                      automation approach offers a promising tool for high
                      throughput root seedling screening in natural soil
                      environments, along with an opportunity to link the results
                      with physiological measurements on root performance. This
                      work was partly financially supported by BASF.},
      month         = {Nov},
      date          = {2023-11-07},
      organization  = {7th conference on cereal biotechnology
                       and breeding, wernigerode (Germany), 7
                       Nov 2023 - 9 Nov 2023},
      subtyp        = {Invited},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/1020589},
}