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@INPROCEEDINGS{Jindamol:1020942,
      author       = {Jindamol, Hathairut and Rascher, Uwe and Thiele, Björn and
                      Wuyts, Nathalie and Linow, Mark Müller and Mosaleeyanon,
                      Kriengkrai and Chutimanukul, Panita and Toojinda, Theerayut
                      and Junker-Frohn, Laura Verana},
      title        = {{D}evelopment of a {N}on-{D}estructive {Q}uantification
                      {M}ethod for the {S}econdary {M}etabolites {E}ugenol and
                      {M}ethyl eugenol in the {M}edicinal {P}lant {H}oly
                      {B}asilbased on {H}yperspectral {R}eflectance},
      reportid     = {FZJ-2024-00411},
      year         = {2023},
      abstract     = {In the industrial production of essential oils from
                      medicinal plants, the quantity of secondary metabolites is
                      relevant the quality of the essential oil. The secondary
                      metabolite content varies depending on several factors
                      during cultivation. Quantification of secondary metabolites
                      is necessary to set growing conditions and define the
                      optimal harvesting time to obtain the desired quantity of
                      secondary metabolites. Spectral reflectance measurements of
                      plants are a promising technique for increasing the
                      effectiveness of such monitoring, as they can be performed
                      non-destructively, rapidly and in real-time. The purpose of
                      this study is to develop a non-destructive quantification
                      method for eugenol and methyl eugenol, the crucial
                      constituents in the essential oil of Holy basil, using
                      leaf-level hyperspectral data. Point measurements of
                      spectral reflectance in the spectrum range from 350 to 2500
                      nm were collected from a total of 200 leaves sampled from
                      two cultivars, a green and a red cultivar of commercial
                      relevance. Plants were sampled at four developmental stages:
                      vegetative, pre-flowering, flowering, and harvesting, and
                      leaf samples differed in leaf age and position. Eugenol and
                      methyl eugenol content of each leaf sample was analyzed by
                      the standard method of gas chromatography-mass spectrometry
                      (GC-MS). A wide range of eugenol and methyl eugenol
                      concentrations was found among cultivars and stages of
                      development between 140-7500 and 235-11000 μg/gDW,
                      respectively. The resulting dataset including spectral data
                      and target secondary metabolite data was analysed using
                      partial least squares regression (PLSR). The PLSR modeling
                      resulted in a coefficient of determination (R2) of the
                      calibration and validation sets of about 0.5 for both
                      secondary metabolites, with RMSEP of 1.3 and 1.13 in eugenol
                      and methyl eugenol, respectively. The study demonstrated
                      that a non-destructive quantification technique by detecting
                      hyperspectral reflectance can be used for estimating the
                      content of eugenol and methyl eugenol in Holy basil. In the
                      future, the study will be extended to increase the number of
                      data points and include more cultivars and stress conditions
                      to achieve a higher accuracy needed to develop a
                      non-destructive quantification method.},
      month         = {Oct},
      date          = {2023-10-13},
      organization  = {Specialized products from plants and
                       microbes – a natural source for
                       biologically active compounds, Bad
                       Endbach (Germany), 13 Oct 2023 - 15 Oct
                       2023},
      subtyp        = {Other},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/1020942},
}