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@ARTICLE{Maassen:1020959,
      author       = {Maassen, Jessika and Guenther, Rebecca and Hondrich, Timm
                      J. J. and Cepkenovic, Bogdana and Brinkmann, Dominik and
                      Maybeck, Vanessa and Offenhäusser, Andreas and Dittrich,
                      Barbara and Müller, Anna and Skazik-Voogt, Claudia and
                      Kosel, Maximilian and Baum, Christoph and Gutermuth, Angela},
      title        = {{I}n {V}itro {S}imulated {N}euronal {E}nvironmental
                      {C}onditions {Q}ualify {U}mbilical {C}ord {D}erived {H}ighly
                      {P}otent {S}tem {C}ells for {N}euronal {D}ifferentiation},
      journal      = {Stem cell reviews and reports},
      volume       = {19},
      number       = {6},
      issn         = {2629-3269},
      address      = {New York, NY},
      publisher    = {Humana Press},
      reportid     = {FZJ-2024-00427},
      pages        = {1870 - 1889},
      year         = {2023},
      abstract     = {The healing of neuronal injuries is still an unachieved
                      goal. Medicine-based therapies can only extend the survival
                      of patients, but not finally lead to a healing process.
                      Currently, a variety of stem cell-based tissue engineering
                      developments are the subject of many research projects to
                      bridge this gap. As yet, neuronal differentiation of induced
                      pluripotent stem cells (iPS), embryonic cell lines, or
                      neuronal stem cells could be accomplished and produce
                      functional neuronally differentiated cells. However,
                      clinical application of cells from these sources is hampered
                      by ethical considerations. To overcome these hurdles
                      numerous studies investigated the potential of adult
                      mesenchymal stem cells (MSCs) as a potential stem cell
                      source. Adult MSCs have been approved as cellular
                      therapeutical products due to their regenerative potential
                      and immunomodulatory properties. Only a few of these studies
                      could demonstrate the capacity to differentiate MSCs into
                      active firing neuron like cells. With this study we
                      investigated the potential of Wharton's Jelly (WJ) derived
                      stem cells and focused on the intrinsic pluripotent stem
                      cell pool and their potential to differentiate into active
                      neurons. With a comprehensive neuronal differentiation
                      protocol comprised of mechanical and biochemical inductive
                      cues, we investigated the capacity of spontaneously forming
                      stem cell spheroids (SCS) from cultured WJ stromal cells in
                      regard to their neuronal differentiation potential and
                      compared them to undifferentiated spheroids or adherent
                      MSCs. Spontaneously formed SCSs show pluripotent and
                      neuroectodermal lineage markers, meeting the pre-condition
                      for neuronal differentiation and contain a higher amount of
                      cells which can be differentiated into cells whose
                      functional phenotypes in calcium and voltage responsive
                      electrical activity are similar to neurons. In conclusion we
                      show that up-concentration of stem cells from WJ with
                      pluripotent characteristics is a tool to generate neuronal
                      cell replacement.},
      cin          = {IBI-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524) / 5244 - Information Processing in Neuronal
                      Networks (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241 / G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {37093520},
      UT           = {WOS:000976970000001},
      doi          = {10.1007/s12015-023-10538-w},
      url          = {https://juser.fz-juelich.de/record/1020959},
}