TY  - CONF
AU  - Koller, Robert
AU  - Huber, Gregor
AU  - Pflugfelder, Daniel
AU  - van Dusschoten, Dagmar
AU  - Hinz, Carsten
AU  - Schultes, Sina
AU  - Chlubek, Antonia
AU  - Knief, Claudia
AU  - Metzner, Ralf
TI  - Monitoring spatial and temporal carbon dynamics in the plant soil system by co-registration of Magnetic Resonance Imaging and Positron Emission Tomography for image guided sampling
M1  - FZJ-2024-00536
PY  - 2023
AB  - Individual plants vary in their ability to respond to environmental changes. The plastic response of a plant enhances its ability to avoid environmental constraints, and hence supports growth, reproduction, and evolutionary and agricultural success.Major progress in the analysis of above- and belowground processes on individual plants has been made by the application of non-invasive imaging methods including Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET).MRI allows for repetitive measurements of roots growing in soil and facilitates quantification of root system architecture traits in 3D. PET, on the other hand, opens a door to analyze dynamic physiological processes in plants such as long-distance carbon transport in a repeatable manner. Combining MRI with PET enables monitoring of short livedCarbon tracer (11C) allocation along the transport paths (i.e. roots visualized by MRI) into active sink structures.To analyse the link between root-internal C allocation patterns and C metabolism in the rhizosphere, we are combining 11CO2 with stable 13CO2 labelling of plants. Isotope ratio mass spectrometry (IRMS) analyses of rhizosphere soil is applied to link root-internal C allocation patterns with distribution of 13C in the rhizosphere soil. The metabolically active rhizosphere organisms are subsequently identified based on DNA 13C stable isotope probing.In our presentation we will highlight our approaches for gathering quantitative data from both image-based technologies in combination with destructive analysis that provides insights into the functioning and dynamics of C transport processes in the plant-soil system.
T2  - EGU General Assembly 2023
CY  - 24 Apr 2023 - 28 Apr 2023, Vienna (Austria)
Y2  - 24 Apr 2023 - 28 Apr 2023
M2  - Vienna, Austria
LB  - PUB:(DE-HGF)6
DO  - DOI:10.5194/egusphere-egu23-13308
UR  - https://juser.fz-juelich.de/record/1021082
ER  -