%0 Journal Article
%A Lemke, Moritz
%A Reiners, Jens
%A Smits, Sander H. J.
%A Lakomek, Nils
%A Groth, Georg
%T Functional reconstitution and structural characterization of the plant hormone receptor ETR1 in lipid nanodiscs
%J Chemical communications
%V 59
%N 61
%@ 0022-4936
%C Cambridge
%I Soc.
%M FZJ-2024-00739
%P 9344 - 9347
%D 2023
%X The plant hormone receptor ETR1 regulates many highly relevant agronomic processes. Today, significant functional and structural questions remain unanswered regarding its multi-pass transmembrane sensor domain able to bind and respond to the gaseous plant hormone ethylene at femtomolar concentrations. A significant reason for this is the lack of structural data on full-length ETR1 in a lipid environment. Herein, we present the functional reconstitution of recombinant full-length ETR1 purified and solubilized from a bacterial host into lipid nanodiscs, allowing the study of the purified plant receptor for the first time in a detergent-free membrane-like environment.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 37435887
%U <Go to ISI:>//WOS:001027863000001
%R 10.1039/D3CC02619A
%U https://juser.fz-juelich.de/record/1021443