TY  - JOUR
AU  - Lemke, Moritz
AU  - Reiners, Jens
AU  - Smits, Sander H. J.
AU  - Lakomek, Nils
AU  - Groth, Georg
TI  - Functional reconstitution and structural characterization of the plant hormone receptor ETR1 in lipid nanodiscs
JO  - Chemical communications
VL  - 59
IS  - 61
SN  - 0022-4936
CY  - Cambridge
PB  - Soc.
M1  - FZJ-2024-00739
SP  - 9344 - 9347
PY  - 2023
AB  - The plant hormone receptor ETR1 regulates many highly relevant agronomic processes. Today, significant functional and structural questions remain unanswered regarding its multi-pass transmembrane sensor domain able to bind and respond to the gaseous plant hormone ethylene at femtomolar concentrations. A significant reason for this is the lack of structural data on full-length ETR1 in a lipid environment. Herein, we present the functional reconstitution of recombinant full-length ETR1 purified and solubilized from a bacterial host into lipid nanodiscs, allowing the study of the purified plant receptor for the first time in a detergent-free membrane-like environment.
LB  - PUB:(DE-HGF)16
C6  - 37435887
UR  - <Go to ISI:>//WOS:001027863000001
DO  - DOI:10.1039/D3CC02619A
UR  - https://juser.fz-juelich.de/record/1021443
ER  -