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@ARTICLE{Lemke:1021443,
author = {Lemke, Moritz and Reiners, Jens and Smits, Sander H. J. and
Lakomek, Nils and Groth, Georg},
title = {{F}unctional reconstitution and structural characterization
of the plant hormone receptor {ETR}1 in lipid nanodiscs},
journal = {Chemical communications},
volume = {59},
number = {61},
issn = {0022-4936},
address = {Cambridge},
publisher = {Soc.},
reportid = {FZJ-2024-00739},
pages = {9344 - 9347},
year = {2023},
abstract = {The plant hormone receptor ETR1 regulates many highly
relevant agronomic processes. Today, significant functional
and structural questions remain unanswered regarding its
multi-pass transmembrane sensor domain able to bind and
respond to the gaseous plant hormone ethylene at femtomolar
concentrations. A significant reason for this is the lack of
structural data on full-length ETR1 in a lipid environment.
Herein, we present the functional reconstitution of
recombinant full-length ETR1 purified and solubilized from a
bacterial host into lipid nanodiscs, allowing the study of
the purified plant receptor for the first time in a
detergent-free membrane-like environment.},
cin = {IBI-7},
ddc = {540},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524) / DFG project 267205415 - SFB 1208: Identität
und Dynamik von Membransystemen - von Molekülen bis zu
zellulären Funktionen (267205415)},
pid = {G:(DE-HGF)POF4-5241 / G:(GEPRIS)267205415},
typ = {PUB:(DE-HGF)16},
pubmed = {37435887},
UT = {WOS:001027863000001},
doi = {10.1039/D3CC02619A},
url = {https://juser.fz-juelich.de/record/1021443},
}