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@ARTICLE{Grg:10219,
author = {Görg, B. and Qvartskhava, N. and Bidmon, H.-J. and
Palomero-Gallagher, N. and Kircheis, G. and Zilles, K. and
Häussinger, D.},
title = {{O}xidative stress markers in the brain of patients with
cirrhosis and hepatic encephalopathy},
journal = {Hepatology},
volume = {52},
issn = {0270-9139},
address = {New York [u.a.]},
publisher = {Wiley Interscience},
reportid = {PreJuSER-10219},
pages = {256 - 265},
year = {2010},
note = {This Study was Supported by Deutsche Forschungsgemeinschaft
through Sonderforschungsbereich 575 "Experimentelle
Hepatologle" (Dusseldorf).},
abstract = {Cell culture studies and animal models point to an
important role of oxidative/nitrosative stress in the
pathogenesis of cerebral ammonia toxicity. However, it is
unknown whether oxidative/nitrosative stress in the brain is
also characteristic of hepatic encephalopathy (HE) in
humans. We therefore analyzed post mortem cortical brain
tissue samples from patients with cirrhosis dying with or
without HE in comparison with brains from patients without
liver disease. Significantly elevated levels of protein
tyrosine-nitrated proteins, heat shock protein-27, and
8-hydroxyguanosine as a marker for RNA oxidation were found
in the cerebral cortex of HE patients, but not of patients
with cirrhosis but without HE. Glutamine synthetase (GS)
activity was significantly decreased, whereas GS protein
expression was not significantly affected. Protein
expression of the glutamate/aspartate cotransporter was
up-regulated in HE, whereas protein expression of neuronal
and inducible nitric oxide synthases, manganese-dependent
and copper/zinc-dependent superoxide dismutase, and glial
glutamate transporter-1 were not significantly increased.
CONCLUSION: These data indicate that HE in patients with
cirrhosis is associated with oxidative/nitrosative stress,
protein tyrosine nitration, and RNA oxidation, suggesting a
role of oxidative stress in the pathogenesis of HE in
patients with cirrhosis.},
keywords = {Adult / Aged / Amino Acid Transport System X-AG: analysis /
Amino Acid Transport System X-AG: metabolism / Biological
Markers: analysis / Biological Markers: metabolism /
Cerebral Cortex: chemistry / Cerebral Cortex: metabolism /
Excitatory Amino Acid Transporter 2: analysis / Excitatory
Amino Acid Transporter 2: metabolism / Female /
Glutamate-Ammonia Ligase: analysis / Glutamate-Ammonia
Ligase: metabolism / Guanosine: analogs $\&$ derivatives /
Guanosine: analysis / Guanosine: metabolism / HSP27
Heat-Shock Proteins: analysis / HSP27 Heat-Shock Proteins:
metabolism / Hepatic Encephalopathy: etiology / Hepatic
Encephalopathy: metabolism / Humans / Liver Cirrhosis:
complications / Male / Middle Aged / Nitrates: analysis /
Nitrates: metabolism / Oxidative Stress / RNA: analysis /
RNA: metabolism / Tyrosine: analysis / Tyrosine: metabolism
/ Amino Acid Transport System X-AG (NLM Chemicals) /
Biological Markers (NLM Chemicals) / Excitatory Amino Acid
Transporter 2 (NLM Chemicals) / HSP27 Heat-Shock Proteins
(NLM Chemicals) / Nitrates (NLM Chemicals) / Guanosine (NLM
Chemicals) / 8-hydroxyguanosine (NLM Chemicals) / Tyrosine
(NLM Chemicals) / RNA (NLM Chemicals) / Glutamate-Ammonia
Ligase (NLM Chemicals) / J (WoSType)},
cin = {INM-2 / JARA-BRAIN},
ddc = {610},
cid = {I:(DE-Juel1)INM-2-20090406 / $I:(DE-82)080010_20140620$},
pnm = {Funktion und Dysfunktion des Nervensystems (FUEK409) /
89571 - Connectivity and Activity (POF2-89571)},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-HGF)POF2-89571},
shelfmark = {Gastroenterology $\&$ Hepatology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20583283},
pmc = {pmc:PMC3395472},
UT = {WOS:000279409200028},
doi = {10.1002/hep.23656},
url = {https://juser.fz-juelich.de/record/10219},
}