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@PHDTHESIS{Kuppe:1022314,
author = {Kuppe, Christian W.},
title = {{R}hizosphere models and their application to resource
uptake efficiency},
school = {RWTH Aachen},
type = {Dissertation},
publisher = {RWTH Aachen University},
reportid = {FZJ-2024-01432},
pages = {1 Online-Ressource : Illustrationen},
year = {2023},
note = {Dissertation, RWTH Aachen, 2023},
abstract = {The efficient acquisition of finite and plant
growth-limiting resources becomes increasingly important in
sustainable agriculture and crop production. The rhizosphere
as root-soil interface and its traits are critical for plant
nutrition. Thus, I aim at discovering traits and mechanisms
for efficient nutrient uptake via rhizosphere modeling.
Rhizosphere models are interdisciplinary. They build on soil
models and encompass a collective equation system of
biological, physical, and chemical concepts. Such concepts
and underlying assumptions are fundamental to simulation
results and their interpretation. Hence, in the first part
of the thesis, I comprehensively analyze how the rhizosphere
has been modeled so far. Nutrient uptake calculated from
rhizosphere models affects the entire plant. I compared
numerical methods for calculating uptake, since for root
architecture models with a large number of root segments,
each with its own soil conditions with a priori unknown
concentration profiles, such methods need to be reliable,
fast, and accurate. The hitherto de facto standard methods
for single ion transport in radial rhizosphere models,
Crank-Nicolson and explicit Euler, are not universally
applicable for the wide range of parameter values. I
recommend adaptive stiff or Runge-Kutta methods with
higher-order spatial discretizations. In the second part of
the thesis, I develop two novel models to simulate the
uptake of the essential nutrients, phosphorus (P) and
nitrogen (N). This mechanistic modeling addresses how plant
traits influence uptake efficiency. I explain how upland
rice can efficiently grow on strongly sorbing soils with low
plant-available P and how root-exudation of biological
nitrification inhibitors (BNIs) can facilitate N uptake
efficiency and reduce N loss to the environment. The
mechanisms behind efficient P uptake on strongly sorbing
soils, and the role of different root classes, were not well
understood. Fine lateral roots are metabolically low-cost
and make up a large proportion of the root system, but their
sole uptake strength is low. Models typically underestimated
P uptake, which restrained targeted trait selection.
However, the new P-pH model agrees with the observed plant
P-uptake. The model allows for fast- and slowly reacting P
depending on root-induced pH change, different root classes,
and root morphology. The pH value (in the initially acidic
soil) increases throughout the rhizosphere by acid-base
diffusion, forming a P solubilization zone around the root.
However, this solubilized P diffuses to the root too slowly.
The results of the data-driven modeling convey to breeders
the importance of solubilization and fine hairy lateral
roots as integrative phenotype, i.e. in combination with
other traits, because fine roots are most beneficial for P
uptake in the vicinity of thicker roots due to their greater
solubilization of P. BNIs have been suggested as strategy
for improving N uptake and reducing environmental N
pollution. Not all plants exude BNIs, and their importance
to plants is questionable. With the new N-BNI model, I
investigate the efficacy of BNIs and explain under which
conditions BNIs are beneficial. The benefit of nitrification
inhibition for uptake strongly depends on the availability
of soil N-forms. BNIs are only beneficial for plant-N uptake
when it is not impaired by lower nitrate production over the
growth period. If nitrate availability is low, nitrification
would be beneficial for uptake. The model indicates that the
same N uptake can be achieved with reduced fertilizer
application due to reduced nitrification and, therefore,
reduced N loss. As mode of action, the sensitivity analysis
suggests bactericidal exudates rather than bacteriostatic
ones. Selection for BNI exudation should be accompanied by
improved ammonium uptake. In conclusion, the rhizosphere
models in this thesis enabled the identification of traits
for P- and N-efficient plants. The results of the P-pH
modeling are already used by a plant breeder developing new
rice lines. Future studies on BNIs are required to consider
rhizosphere conditions since the ecological and
plant-physiological benefits of BNIs are distinct.},
keywords = {Hochschulschrift (Other) / Rhizosphere modeling ; nitrogen
; nitrate ; ammonium ; N uptake ; phosphorus ; P uptake ;
soil pH ; phosphate ; solubilization ; upscaling ; BNI ;
radial solute transport; NUE; uptake efficiency ; bacteria ;
nitrification ; inhibition ; upland rice ; roots ; numerical
methods ; Wurzel ; N-Aufnahme ; Nitrifizierung ; P-Aufnahme
(Other)},
cin = {IBG-2 / IBG-1 / IBG-4},
cid = {I:(DE-Juel1)IBG-2-20101118 / I:(DE-Juel1)IBG-1-20101118 /
I:(DE-Juel1)IBG-4-20200403},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2023-11292},
url = {https://juser.fz-juelich.de/record/1022314},
}