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001023000 037__ $$aFZJ-2024-01589
001023000 1001_ $$0P:(DE-Juel1)130542$$aBiehl, Ralf$$b0$$eCorresponding author
001023000 1112_ $$aAntibodies in Solution: a LINXS - NIST Webinar Series$$cOnline$$wSweden
001023000 245__ $$aDomain Motions: Protein Dynamics seen by Neutron Spinecho Spectroscopy$$f2024-02-07 - 
001023000 260__ $$c2024
001023000 3367_ $$033$$2EndNote$$aConference Paper
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001023000 3367_ $$2BibTeX$$aINPROCEEDINGS
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001023000 520__ $$aThe biological function of proteins is often related to configurational changes and large-scale domain motions, which are induced or suppressed by the binding of a substrate or due to cosolvents. Domain motions can be related to soft hinges, flexible linker regions or -as in the case of intrinsically disordered proteins- be native to proteins without secondary or tertiary structure. These large-scale domain motions in solution cannot be observed by X-ray crystallography or NMR spectroscopy. Small angle scattering (SAS) by X-rays or neutrons in combination with neutron spin echo spectroscopy (NSE) in solution can be used to observe configurational changes and equilibrium dynamics between functional domains on 1-300 nanosecond timescale.I will present examples for different types of motions related to the structure of proteins and bioconjugates. Thermal unfolded Ribonuclease A shows polymer like dynamics despite the 4 disulfide bonds restricting the degrees of freedom. Prior to full unfolding the protein unfolding dynamics is observed. Polyelectrolytes have structural and dynamical similarities with IDP. The domain protein Phosphoglycerate kinase shows a hinge motion between the main domains related to function. PEGylation seems not to influence this domain motion but adds additional internal dynamics in the protein-polymer complex. Antibodies present a strong dynamics due to the short linkers connecting the Fc with the Fab domains. The observed dynamics is related to internal forces, solvent friction and the role of charge screening.
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001023000 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
001023000 65017 $$0V:(DE-MLZ)GC-130-2016$$2V:(DE-HGF)$$aHealth and Life$$x0
001023000 8564_ $$uhttps://www.linxs.se/events/2024/02/07/antibodies-in-solution-ralf-biehl-linxs-event
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001023000 9131_ $$0G:(DE-HGF)POF4-524$$1G:(DE-HGF)POF4-520$$2G:(DE-HGF)POF4-500$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-5241$$aDE-HGF$$bKey Technologies$$lNatural, Artificial and Cognitive Information Processing$$vMolecular and Cellular Information Processing$$x3
001023000 9141_ $$y2024
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