001025088 001__ 1025088 001025088 005__ 20250203103351.0 001025088 0247_ $$2doi$$a10.1093/micmic/ozad067.521 001025088 0247_ $$2ISSN$$a1079-8501 001025088 0247_ $$2ISSN$$a1431-9276 001025088 0247_ $$2ISSN$$a1435-8115 001025088 0247_ $$2datacite_doi$$a10.34734/FZJ-2024-02676 001025088 037__ $$aFZJ-2024-02676 001025088 082__ $$a500 001025088 1001_ $$0P:(DE-Juel1)177744$$aBerkamp, Sabrina$$b0$$ufzj 001025088 245__ $$aHigh-Throughput Correlative Light and Cryo-Electron Microscopy Pipeline Using PRIMO Micropatterning, CERES Ice Shield and the METEOR In-Chamber Fluorescence Light Microscope 001025088 260__ $$aOxford$$bOxford University Press$$c2023 001025088 3367_ $$2DRIVER$$aarticle 001025088 3367_ $$2DataCite$$aOutput Types/Journal article 001025088 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1712833405_20085 001025088 3367_ $$2BibTeX$$aARTICLE 001025088 3367_ $$2ORCID$$aJOURNAL_ARTICLE 001025088 3367_ $$00$$2EndNote$$aJournal Article 001025088 520__ $$aCorrelative light and cryo-electron microscopy (CLEM) can be used to study macromolecular structures in vitreous state of the complex environment of the cell. The associated workflow, however, is still cumbersome and low throughput. It includes many steps and is therefore prone to ice contamination, cracked lamellae or devitrification all of which can result in sample loss. We have implemented several technological improvements to the workflow resulting in a much simpler and higher throughput method, yielding more lamellae with reduced ice contamination. 001025088 536__ $$0G:(DE-HGF)POF4-5352$$a5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535)$$cPOF4-535$$fPOF IV$$x0 001025088 536__ $$0G:(DE-HGF)POF4-5241$$a5241 - Molecular Information Processing in Cellular Systems (POF4-524)$$cPOF4-524$$fPOF IV$$x1 001025088 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de 001025088 7001_ $$0P:(DE-HGF)0$$aSmeets, Marit$$b1 001025088 7001_ $$0P:(DE-HGF)0$$aCaignard, Alexane$$b2 001025088 7001_ $$0P:(DE-HGF)0$$aJani, Riddhi$$b3 001025088 7001_ $$0P:(DE-HGF)0$$aDaviran, Deniz$$b4 001025088 7001_ $$0P:(DE-Juel1)173949$$aSachse, Carsten$$b5$$eCorresponding author 001025088 773__ $$0PERI:(DE-600)1481716-0$$a10.1093/micmic/ozad067.521$$gVol. 29, no. 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