TY  - JOUR
AU  - Berkamp, Sabrina
AU  - Smeets, Marit
AU  - Caignard, Alexane
AU  - Jani, Riddhi
AU  - Daviran, Deniz
AU  - Sachse, Carsten
TI  - High-Throughput Correlative Light and Cryo-Electron Microscopy Pipeline Using PRIMO Micropatterning, CERES Ice Shield and the METEOR In-Chamber Fluorescence Light Microscope
JO  - Microscopy and microanalysis
VL  - 29
IS  - Supplement_1
SN  - 1079-8501
CY  - Oxford
PB  - Oxford University Press
M1  - FZJ-2024-02676
SP  - 1027 - 1028
PY  - 2023
AB  - Correlative light and cryo-electron microscopy (CLEM) can be used to study macromolecular structures in vitreous state of the complex environment of the cell. The associated workflow, however, is still cumbersome and low throughput. It includes many steps and is therefore prone to ice contamination, cracked lamellae or devitrification all of which can result in sample loss. We have implemented several technological improvements to the workflow resulting in a much simpler and higher throughput method, yielding more lamellae with reduced ice contamination.
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1093/micmic/ozad067.521
UR  - https://juser.fz-juelich.de/record/1025088
ER  -